Recombinant Human Complement Factor D/CFD (His Tag)

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HEK293表达系统补体替代途径研究抗体药物靶点研究

Product no.93773ES Sizes4 options SourceYeasen · Official

Cat. No. 93773ES25

现货 ¥1,195.00

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93773ES25 25 μg ¥1,195.00 现货 Enquire

93773ES60 100 μg ¥4,445.00 现货 Enquire

93773ES76 500 μg ¥14,855.00 现货 Enquire

93773ES80 1 mg ¥29,715.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93773ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 补体替代途径研究研究Complement Factor D/CFD在补体替代途径中的催化作用 抗体药物靶点研究作为靶点蛋白用于相关抗体药物的筛选与开发

适用物种种属人

检测方法 SDS-PAGE HPLC LAL法 ELISA Tris-Bis PAGE

生物特性参数 蛋白类型重组蛋白 种属来源人 生物活性描述丝氨酸蛋白酶，催化补体替代途径的初始蛋白水解步骤；ELISA测得Anti-Complement Factor D Antibody（hFc Tag）的EC50为16 ng/ml。 应用产品数据 产品别称Adipsin C3 convertase activator Complement factor D CFD PFD DF ADN FACTOR D AMBP-1 EC 3.4.21 EC 3.4.21.46

样本类型 重组蛋白

技术参数规格高纯度、高活性、低内毒素、高批间一致性 纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Approximately 25.5 kDa. Due to glycosylation the protein migrates to 26-27 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性【ELISA Data】Immobilized Human Complement Factor D His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Complement Factor D Antibody hFc Tag with the EC50 of 16ng/ml determined by ELISA. 储存条件-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。 表达系统HEK293 标签C-His 表达序列Ile26-Ala253 AccessionP00746.1 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 复溶浓度>100 μg/mL