Recombinant Human E-selectin/CD62E (His-Avi Tag)

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HEK293表达系统抗体药物靶点研究炎症机制研究

Product no.93777ES Sizes4 options SourceYeasen · Official

Cat. No. 93777ES25

现货 ¥565.00

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93777ES25 25 μg ¥565.00 现货 Enquire

93777ES60 100 μg ¥2,115.00 现货 Enquire

93777ES76 500 μg ¥7,065.00 现货 Enquire

93777ES80 1 mg ¥13,255.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93777ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 抗体药物靶点研究作为重组靶点蛋白用于抗体筛选与药物开发 炎症机制研究用于研究E-Selectin介导的白细胞黏附与炎症过程

适用物种种属人

检测方法 ELISA SDS-PAGE HPLC LAL法

生物特性参数 蛋白类型重组蛋白；E-Selectin/CD62E，Selectin家族细胞表面糖蛋白 种属来源Human（人） 生物活性描述介导炎症过程中流动白细胞与血管壁的黏附；ELISA测定Anti-E-Selectin Antibody hFc Tag的EC50为8.0 ng/ml 应用抗体药物靶点；炎症机制研究

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC 内毒素水平< 1.0 EU per μg by the LAL method 分子量Approximately 61.6 kDa; due to glycosylation the protein migrates to 80-100 kDa based on Tris-Bis PAGE result 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性ELISA: EC50 = 8.0 ng/ml for Anti-E-Selectin Antibody hFc Tag determined by ELISA 储存条件-25 ~ -15℃保存，有效期1年；复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期 表达系统HEK293 标签C-His-Avi AccessionP16581 表达序列Trp22-Pro556 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。