Recombinant Human IL-15RA&IL-15 Protein,His Tag

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HEK293表达系统

Product no.93831ES Sizes3 options SourceYeasen · Official

Cat. No. 93831ES60

现货 $3,985.00

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Cat.No Spec Price Status

93831ES60 100 μg $3,985.00 现货 Enquire

93831ES76 500 μg $13,255.00 现货 Enquire

93831ES25 25 μg $1,195.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No93831ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

适用物种种属人

检测方法 SDS-PAGE HPLC ELISA SPR 鲎试剂法

生物特性参数 蛋白类型重组蛋白 种属来源人 生物活性描述ELISA: Immobilized Human IL-15RA&IL-15 His Tag at 1μg/ml. Dose response curve for Human IL-2 R beta&IL-2 R gamma hFc Tag with EC50 of 72.0ng/ml. SPRHuman IL-2 R beta&IL-2 R gamma hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15 His Tag with an affinity constant of 0.084 nM.

技术参数纯度>95% as determined by SDS-PAGE and HPLC 内毒素水平<1.0 EU per μg by LAL method 分子量Predicted MW 23.8 kDa; due to glycosylation migrates to 26-28 kDa & 30-32 kDa on Tris-Bis PAGE 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose added as protectant before lyophilization. 活性ELISA: EC50=72.0 ng/ml; SPR: affinity constant=0.084 nM 储存条件-25~-15℃保存，有效期1年；复溶后，-85~-65℃保存，3个月 表达系统HEK293 标签C-His 表达序列Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15) 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 蛋白登录号Q13261-1(IL-15RA)&P40933-1(IL-15)