品牌翌圣生物 Yeasen
货号 / 型号93834ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293表达系统
应用方法
抗体药物靶点蛋白靶点研究和药物筛选
适用物种种属
Human
检测方法
SDS-PAGE
HPLC
LAL法
ELISA
SPR
生物特性参数
蛋白类型重组蛋白
种属来源Human
生物活性描述【ELISA Data】Immobilized Human TRAIL
No Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TRAIL R4
His Tag with the EC50 of 76.1ng/ml determined by ELISA.【SPR Data】Human TRAIL R4
His Tag immobilized on CM5 Chip can bind Human TRAIL
No Tag with an affinity constant of 0.13 nM as determined in SPR assay.
应用抗体药物靶点蛋白
产品别称CD264
RSF10D
TRAILR4
DCR2
TRUNDD
TNFRSF10D
技术参数
纯度>95% as determined by SDS-PAGE and HPLC.
内毒素水平<1.0 EU per μg by the LAL method.
分子量The protein has a predicted MW of 19.4 kDa. Due to glycosylation
the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
活性EC50 of 76.1ng/ml determined by ELISA; affinity constant of 0.13 nM determined by SPR.
储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。
表达系统HEK293
标签C-His-Avi
表达序列Ala56-His211
外观Lyophilized Powder
蛋白数据库编号Q9UBN6
复溶方法开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
实验类型
ELISA测定生物活性
SPR测定分子间亲和力
SDS-PAGE测定纯度
HPLC测定纯度
LAL法测定内毒素水平