品牌翌圣生物 Yeasen
货号 / 型号93941ES
规格高纯度、高活性、低内毒素、高批间一致性
产地翌圣生物科技(上海)股份有限公司
适用物种种属
人
检测方法
LAL法
SDS-PAGE
HPLC
Tris-Bis PAGE
SPR
生物特性参数
蛋白类型Recombinant Human KIR3DL3 Protein
种属来源Human
生物活性描述SPR Data: Human B7-H7
hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3
His Tag with an affinity constant of 334 nM as determined in SPR assay.
技术参数
纯度> 95% as determined by SDS-PAGE and HPLC.
内毒素水平< 1.0 EU per μg by the LAL method.
分子量The protein has a predicted MW of 35.3 kDa. Due to glycosylation
the protein migrates to 48-52 kDa based on Tris-Bis PAGE result.
制剂Lyophilized from 0.22μm filtered solution in 20mM Tris
150mM NaCl
0.2M L-arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization.
活性SPR Data: Human B7-H7
hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3
His Tag with an affinity constant of 334 nM.
储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。
表达序列Glu26-Leu322
表达系统HEK293
标签C-His-Avi
Accession号Q8N743
产品别称CD158Z
KIR44
KIR3DL7
KIR3DL3
复溶方法开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。
外观Lyophilized Powder
实验类型
SPR实验检测分子结合亲和力
LAL实验检测内毒素水平
SDS-PAGE检测蛋白纯度
HPLC检测蛋白纯度
Tris-Bis PAGE检测蛋白分子量