Recombinant Biotinylated Human GFRAL/GFR alpha-like Protein,His-Avi Tag

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HEK293细胞表达系统AviTag生物素标记技术抗体药物靶点研究ELISA检测重组蛋白研究

Product no.93950ES Sizes3 options SourceYeasen · Official

Cat. No. 93950ES25

现货 ¥2,285.00

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93950ES25 25 μg ¥2,285.00 现货 Enquire

93950ES60 100 μg ¥6,885.00 现货 Enquire

93950ES76 500 μg ¥24,115.00 现货 Enquire

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Specifications

Brand翌圣生物 Yeasen

Cat.No93950ES

Purity> 95% as determined by SDS-PAGE and HPLC.

Specification100 μg

Origin翌圣生物科技(上海)股份有限公司

复溶方法 开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。

内毒素 < 1.0 EU per μg by the LAL method.

种属 Human

产品别称 GFR alpha-like GFRAL GRAL C6orf144

制剂 Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

标签 C-His-Avi

纯度 > 95% as determined by SDS-PAGE and HPLC.

Accession Q6UXV0

生物活性 【ELISA Data】Immobilized Human GDF15 His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GFRAL His Tag with the EC50 of 89.1ng/ml determined by ELISABlockingData:Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15 His TagBiotinylated Human GFRAL His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.

表达序列 Ser19-Glu351

表达系统 HEK293

分子量 The protein has a predicted MW of 40.7 kDa. Due to glycosylation the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.

外观 Lyophilized Powder

技术平台 HEK293细胞表达系统 AviTag生物素标记技术

应用方法 抗体药物靶点研究用于GFRAL/GDF15相关抗体药物靶点筛选与功能研究 ELISA检测用于配体-受体结合及阻断分析 重组蛋白研究用于蛋白活性、相互作用及机制研究

适用物种种属人

检测方法 ELISA（酶联免疫吸附测定） SDS-PAGE（SDS-聚丙烯酰胺凝胶电泳） Tris-Bis PAGE HPLC（高效液相色谱） LAL（鲎试剂）法

生物特性参数 蛋白类型重组蛋白（生物素化） 种属来源Human 生物活性描述ELISA Data: Immobilized Human GDF15 His Tag at 1μg/ml Biotinylated Human GFRAL His Tag dose response curve EC5089.1 ng/ml; Blocking Data: Anti-GDF15 Antibody inhibited GDF15:GFRAL binding with IC50=40.8 ng/ml 应用抗体药物靶点蛋白；ELISA配受体结合与阻断分析 UniProt编号Q6UXV0 产品别称GFR alpha-like GFRAL GRAL C6orf144

样本类型 重组蛋白

技术参数浓度复溶浓度建议超过100 μg/mL 纯度>95% as determined by SDS-PAGE and HPLC 内毒素水平< 1.0 EU per μg by LAL method 分子量predicted MW 40.7 kDa；due to glycosylation migrates to 48-62 kDa by Tris-Bis PAGE 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4); normally 8% trehalose added as protectant before lyophilization 活性ELISA结合EC50=89.1 ng/ml；ELISA阻断IC50=40.8 ng/ml 储存条件-25 ~ -15℃保存，有效期1年；复溶后无菌条件下 -85 ~ -65℃保存，3个月有效期 表达系统HEK293 标签C-His-Avi 表达序列Ser19-Glu351 复溶方法开盖前先离心；用蒸馏水复溶，复溶浓度超过100 μg/mL；建议分装避免反复冻融 外观Lyophilized Powder