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Product data sheet PD-94027ES
Recombinant SARS-COV-2 Spike RBD Protein, hFc Tag Recombinant SARS-COV-2 Spike RBD Protein, hFc Tag
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Recombinant SARS-COV-2 Spike RBD Protein, hFc Tag

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HEK293表达系统中和抗体疗法疫苗研发抗病毒治疗血清中和抗体检测
Product no.94027ES Sizes4 options SourceYeasen · Official
Cat. No. 94027ES25
现货 ¥1,185.00
Qty 1
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Cat.No Spec Price Status
94027ES25 25 μg ¥1,185.00 现货 Enquire
94027ES60 100 μg ¥4,285.00 现货 Enquire
94027ES76 500 μg ¥16,885.00 现货 Enquire
94027ES80 1 mg ¥31,255.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No94027ES
Specification高纯度、高活性、低内毒素、高批间一致性
Origin翌圣生物科技(上海)股份有限公司
技术平台 HEK293表达系统
应用方法 中和抗体疗法RBD是中和抗体靶点,可抑制SARS-CoV-2与ACE2受体结合 疫苗研发RBD可作为疫苗免疫原靶点 抗病毒治疗RBD可作为抗病毒治疗靶点 血清中和抗体检测用于检测患者血液中是否存在中和抗体,评估感染后免疫状态
适用物种种属 SARS-CoV-2
检测方法 酶联免疫吸附测定(ELISA) 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE) 高效液相色谱(HPLC) 鲎试剂法(LAL) Tris-Bis聚丙烯酰胺凝胶电泳(Tris-Bis PAGE)
生物特性参数 蛋白类型重组蛋白 种属来源SARS-CoV-2(病毒) 生物活性描述【ELISA Data】Immobilized Cynomolgus ACE2 His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. 应用中和抗体疗法、疫苗研发、抗病毒治疗、血清中和抗体检测
样本类型 重组蛋白
技术参数 纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Approximately 50.7 kDa. Due to glycosylation the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 活性【ELISA Data】Immobilized Cynomolgus ACE2 His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for SARS-COV-2 Spike RBD hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. 储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。 表达系统HEK293 标签C-hFc 表达序列Arg319-Phe541 复溶方法开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。 外观Lyophilized Powder AccessionQHD43416.1
实验类型 ELISA结合实验测定SARS-CoV-2 Spike RBD蛋白与Cynomolgus ACE2的结合活性,EC50为5.2 ng/ml SDS-PAGE纯度检测蛋白纯度鉴定 HPLC纯度检测蛋白纯度鉴定 LAL内毒素检测内毒素水平测定 Tris-Bis PAGE分子量检测糖基化后分子量迁移范围鉴定

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