Recombinant SARS-COV-2 Spike S1(Delta B.1.617.2) Protein, His Tag

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HEK293细胞新冠相关研究

Product no.94042ES Sizes3 options SourceYeasen · Official

Cat. No. 94042ES60

现货 ¥4,225.00

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94042ES60 100 μg ¥4,225.00 现货 Enquire

94042ES76 500 μg ¥16,885.00 现货 Enquire

94042ES80 1 mg ¥31,255.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No94042ES

Specification100 μg / 500 μg / 1 mg

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞

应用方法 新冠相关研究用于SARS-CoV-2 Spike S1蛋白功能研究及疫苗/药物开发

适用物种种属 SARS-CoV-2

检测方法 ELISA Tris-Bis PAGE HPLC LAL法

生物特性参数 蛋白类型重组蛋白 种属来源SARS-CoV-2 生物活性描述ELISA Data: Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2) His Tag at 0.5μg/ml (100 μl/Well) on the plate. Dose response curve for Human ACE2 hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. 应用新冠相关研究

技术参数 表达系统HEK293 Cells 标签His tag at the C-terminal 纯度> 95% as determined by Tris-Bis PAGE and HPLC 内毒素水平< 1.0 EU per 1μg of the protein by the LAL method 分子量Approximately 75.92 kDa. Due to glycosylation the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性ELISA Data: Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2) His Tag at 0.5μg/ml (100 μl/Well) on the plate. Dose response curve for Human ACE2 hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. 储存条件-25~-15℃ for 1 year; after reconstitution 2~8°C for 1 month -25~-15°C for 3 months; avoid repeated freeze-thaw cycles. 外观冻干粉 表达区间Gln14-Arg683(T19R G142D E156G HR157-158 del L452R T478K D614G P681R) 复溶方法Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water Uniprot IDP0DTC2 产品别称S1 protein; Spike protein S1; Spike S1 protein; S glycoprotein Subunit1