品牌翌圣生物 Yeasen
货号 / 型号94042ES
规格100 μg / 500 μg / 1 mg
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293细胞
应用方法
新冠相关研究用于SARS-CoV-2 Spike S1蛋白功能研究及疫苗/药物开发
适用物种种属
SARS-CoV-2
检测方法
ELISA
Tris-Bis PAGE
HPLC
LAL法
生物特性参数
蛋白类型重组蛋白
种属来源SARS-CoV-2
生物活性描述ELISA Data: Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2)
His Tag at 0.5μg/ml (100 μl/Well) on the plate. Dose response curve for Human ACE2
hFc Tag with the EC50 of 21.8ng/ml determined by ELISA.
应用新冠相关研究
技术参数
表达系统HEK293 Cells
标签His tag at the C-terminal
纯度> 95% as determined by Tris-Bis PAGE and HPLC
内毒素水平< 1.0 EU per 1μg of the protein by the LAL method
分子量Approximately 75.92 kDa. Due to glycosylation
the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
活性ELISA Data: Immobilized SARS-CoV-2 Spike S1 (Delta B.1.617.2)
His Tag at 0.5μg/ml (100 μl/Well) on the plate. Dose response curve for Human ACE2
hFc Tag with the EC50 of 21.8ng/ml determined by ELISA.
储存条件-25~-15℃ for 1 year; after reconstitution 2~8°C for 1 month
-25~-15°C for 3 months; avoid repeated freeze-thaw cycles.
外观冻干粉
表达区间Gln14-Arg683(T19R
G142D
E156G
HR157-158 del
L452R
T478K
D614G
P681R)
复溶方法Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water
Uniprot IDP0DTC2
产品别称S1 protein; Spike protein S1; Spike
S1 protein; S glycoprotein Subunit1
实验类型
ELISA用于检测蛋白与ACE2的结合活性
Tris-Bis PAGE用于检测蛋白纯度及分子量
HPLC用于检测蛋白纯度
LAL法用于检测内毒素水平
