品牌翌圣生物 Yeasen
货号 / 型号94043ES
规格100 μg / 500 μg / 1 mg
产地翌圣生物科技(上海)股份有限公司
适用物种种属
SARS-CoV-2
检测方法
ELISA
SDS-PAGE
HPLC
鲎试剂法
生物特性参数
蛋白类型重组蛋白
种属来源SARS-CoV-2
生物活性描述Immobilized SARS-CoV-2 Spike RBD (Omicron BQ.1.1)
His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human ACE2
hFc Tag with the EC50 of 15.8ng/ml determined by ELISA.
应用研究SARS-CoV-2 RBD与ACE2的结合
技术参数
表达系统HEK293 Cells
标签His tag at the C-terminal
外观冻干粉
内毒素水平< 1.0 EU per 1μg of the protein by the LAL method
分子量Approximately 25.98 kDa; due to glycosylation
migrates to 35-43 kDa based on Tris-Bis PAGE
表达区间Arg319-Phe541 (G339D
R346T
S371F
S373P
S375F
T376A
D405N
R408S
K417N
N440K
K444T
L452R
N460K
S477N
T478K
E484A
F486V
Q498R
N501Y
Y505H)
制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4)
纯度> 95% as determined by SDS-PAGE and HPLC
活性ELISA EC50 of 15.8 ng/ml for binding to Human ACE2
储存条件-25~-15°C; after reconstitution: 1 month at 2-8°C under sterile conditions
3 months at -25~-15°C under sterile conditions; avoid repeated freeze-thaw cycles
复溶方法Centrifuge tubes before opening. Reconstitute to a concentration more than 100 μg/mL (usually 1 mg/mL) with distilled water.
UniprotP0DTC2
产品别称S protein RBD; S glycoprotein RBD; Spike protein RBD
实验类型
ELISA测定生物活性
