品牌翌圣生物 Yeasen
货号 / 型号94045ES
规格100 μg / 500 μg / 1 mg
产地翌圣生物科技(上海)股份有限公司
技术平台
HEK293细胞表达系统
适用物种种属
SARS-CoV-2
检测方法
ELISA
SDS-PAGE
Tris-Bis PAGE
HPLC
LAL法
生物特性参数
蛋白类型重组蛋白
种属来源SARS-CoV-2
变异株Omicron XBB
生物活性ELISA Data：Immobilized SARS-CoV-2 Spike RBD (Omicron XBB) at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2
hFc Tag with the EC50 of 27.0ng/ml determined by ELISA.
表达区间Arg319-Phe541(G339H
R346T
L368I
S371F
S373P
S375F
T376A
D405N
R408S
K417N
N440K
V445P
G446S
N460K
S477N
T478K
E484A
F486S
F490S
Q498R
N501Y
Y505H)
应用研究SARS-CoV-2 Spike蛋白RBD与ACE2受体的结合
产品别称S protein RBD; S glycoprotein RBD; Spike protein RBD
UniProt IDP0DTC2
样本类型
重组蛋白
技术参数
表达系统HEK293 Cells
标签His tag at the C-terminal
外观冻干粉
内毒素水平< 1.0 EU per 1μg of the protein by the LAL method.
分子量Approximately 25.99 kDa. Due to glycosylation
the protein migrates to 35-43 kDa based on Tris-Bis PAGE result.
制剂Lyophilized from 0.22μm filtered solution in PBS (pH 7.4)
复溶方法Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
纯度> 95% as determined by SDS-PAGE and HPLC.
储存条件The product should be stored at -25~-15℃. 1 month
2 ~8 °C under sterile conditions after reconstitution. 3 months
-25~-15℃ °C under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
活性ELISA检测显示与人ACE2结合的EC50为27.0 ng/ml；浓度等详见生物活性描述。
实验类型
ELISA检测蛋白与ACE2受体的结合活性
SDS-PAGE测定分子量和纯度
Tris-Bis PAGE测定分子量
HPLC测定纯度
LAL法检测内毒素水平
