Recombinant SARS-COV-2 Spike S Trimer Protein, His-Avi Tag

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新冠相关研究

Product no.94049ES Sizes3 options SourceYeasen · Official

Cat. No. 94049ES60

现货 $4,585.00

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94049ES60 100 μg $4,585.00 现货 Enquire

94049ES76 500 μg $16,855.00 现货 Enquire

94049ES80 1 mg $29,855.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No94049ES

Specification100 μg / 500 μg / 1 mg

Origin翌圣生物科技(上海)股份有限公司

应用方法 新冠相关研究S蛋白在诱导中和抗体和T细胞反应及保护性免疫中发挥关键作用

适用物种种属 SARS-CoV-2

检测方法 ELISA Tris-Bis PAGE HPLC LAL

生物特性参数 蛋白类型重组Spike S三聚体蛋白 种属来源SARS-COV-2 生物活性描述ELISA Data: Immobilized SARS-COV-2 Spike S Trimer His Tag at 1μg/ml (100μl/Well). Dose response curve for Human ACE2 hFc Tag with the EC50 of 42.2ng/ml determined by ELISA. 应用S蛋白在诱导中和抗体和T细胞反应及保护性免疫中发挥关键作用

技术参数纯度> 95% as determined by Tris-Bis PAGE and HPLC 内毒素水平< 1.0 EU per 1μg of the protein by the LAL method 分子量Approximately 136.70 kDa. Due to glycosylation the protein migrates to 140-180 kDa based on Tris-Bis PAGE result. 制剂形式Supplied as 0.22μm filtered solution in 10mM PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 储存条件Store at -25~-15℃ for 1 year. After reconstitution: 2~8°C for 1 month -25~-15°C for 3 months. Aliquot to avoid repeated freeze-thaw cycles. 表达系统HEK293 Cells 标签His tag and Avi tag at the C-terminal 外观冻干粉 复溶方法Centrifuge tubes before opening. Reconstitute to concentration more than 100 μg/mL (usually 1 mg/mL). Dissolve in distilled water. 表达序列Val16-Glu1188 Accession号P0DTC2 活性ELISA EC50 = 42.2 ng/ml against Human ACE2