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Product data sheet PD-94114ES
Recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein,His-Avi Tag Recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein,His-Avi Tag
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Recombinant Biotinylated Human GARP&Latent TGF beta 1 Complex Protein,His-Avi Tag

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HEK293细胞表达系统抗体药物靶点研究重组蛋白研究
Product no.94114ES Sizes3 options SourceYeasen · Official
Cat. No. 94114ES60
现货 $9,275.00
Qty 1
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Cat.No Spec Price Status
94114ES60 100 μg $9,275.00 现货 Enquire
94114ES76 500 μg $28,855.00 现货 Enquire
94114ES25 25 μg $2,555.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No94114ES
Specification高纯度、高活性、低内毒素、高批间一致性
Origin翌圣生物科技(上海)股份有限公司
技术平台 HEK293细胞表达系统
应用方法 抗体药物靶点研究作为GARP&Latent TGF beta 1复合物靶点蛋白用于抗体药物开发与筛选 重组蛋白研究用于重组蛋白的功能研究和细胞因子相关实验
适用物种种属 人
检测方法 酶联免疫吸附试验(ELISA) SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE) 高效液相色谱(HPLC) 鲎试验(LAL法)
生物特性参数 蛋白类型重组蛋白/靶点蛋白/抗体药物靶点蛋白 种属来源人(Human) 生物活性描述【ELISA Data】 Immobilized Anti-GARP&Latent TGF beta 1 Antibody hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human GARP&Latent TGF Beta 1 Complex His Tag with the EC50 of 44.0ng/ml determined by ELISA. 应用抗体药物靶点蛋白/重组蛋白研究 产品别称LAP GARP LRRC32 LRRC32&TGF-beta 1
样本类型 重组蛋白
技术参数 纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量The protein has a predicted MW of 70.3 kDa (GARP)&41.4 kDa (Latent TGF beta 1). Due to glycosylation the protein migrates to 73-78 kDa (GARP)&13 kDa&42-45 kDa (Latent TGF beta 1) based on SDS-PAGE result. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 储存条件-25 ~ -15℃保存,收到货之后有效期1年。复溶后,无菌条件下,-85 ~ -65℃保存,3个月有效期。 表达系统HEK293 标签C-His-Avi 表达序列His20-Leu628(GARP)&Leu30-Ser390(Latent TGF beta 1) AccessionQ14392(GARP)&P01137(Latent TGF beta 1) 复溶方法开盖之前,先离心。使用蒸馏水复溶冻干的蛋白质,复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量,避免反复冻融。 外观Lyophilized Powder
实验类型 酶联免疫吸附试验(ELISA)测定生物活性 SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)检测蛋白纯度及分子量 高效液相色谱(HPLC)检测蛋白纯度 鲎试验(LAL法)检测内毒素水平

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