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Product data sheet PD-94229ES
Recombinant Human Kallikrein 5/KLK5 Protein,His Tag Recombinant Human Kallikrein 5/KLK5 Protein,His Tag
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Recombinant Human Kallikrein 5/KLK5 Protein,His Tag

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HEK293表达系统药物靶点研究酶学研究免疫检测
Product no.94229ES Sizes3 options SourceYeasen · Official
Cat. No. 94229ES60
现货 $3,255.00
Qty 1
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Cat.No Spec Price Status
94229ES60 100 μg $3,255.00 现货 Enquire
94229ES76 500 μg $9,625.00 现货 Enquire
94229ES25 25 μg $1,355.00 现货 Enquire
Submit enquiry Price is on application; bulk sizes can be sampled on request.

Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No94229ES
Specification高纯度、高活性、低内毒素、高批间一致性
Origin翌圣生物科技(上海)股份有限公司
技术平台 HEK293表达系统
应用方法 药物靶点研究作为抗体药物靶点蛋白用于药物研发 酶学研究用于激肽释放酶相关蛋白酶的功能研究 免疫检测作为抗原或标准品用于ELISA等免疫检测
适用物种种属 人
检测方法 SDS-PAGE 高效液相色谱 鲎试剂法 酶联免疫吸附试验
生物特性参数 蛋白类型重组蛋白(His标签) 种属来源Human 生物活性描述能够切割荧光底物Boc-VPR-AMC,比活性>200 pmol/min/µg;ELISA显示与Anti-Kallikrein 5抗体结合EC50为9.4 ng/ml 应用抗体药物靶点研究、酶活性测定、免疫检测等
技术参数 纯度>95% as determined by SDS-PAGE and HPLC 内毒素水平<1.0 EU per μg by LAL method 分子量Predicted MW 30.7 kDa; observed 42-48 kDa by SDS-PAGE due to glycosylation 制剂形式Lyophilized from 0.22μm filtered solution in 50mM MES 150mM NaCl (pH 5.5) with 8% trehalose as protectant 活性Specific activity >200 pmol/min/µg (fluorogenic substrate); EC50 9.4 ng/ml by ELISA 储存条件-25~-15℃保存,有效期1年;复溶后-85~-65℃保存,3个月 表达序列Val23-Ser293 表达系统HEK293 标签C-His 外观Lyophilized Powder 复溶方法Centrifuge before opening; reconstitute to >100 μg/ml in 50mM MES 150mM NaCl (pH 5.5) AccessionQ9Y337
实验类型 酶联免疫吸附试验检测蛋白与抗体的结合活性 酶活性测定检测蛋白的蛋白酶催化活性 SDS-PAGE检测蛋白纯度与分子量 高效液相色谱检测蛋白纯度

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