Recombinant Human PGF Protein,hFc Tag

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HEK293表达系统ELISA受体结合研究

Product no.94232ES Sizes3 options SourceYeasen · Official

Cat. No. 94232ES60

现货 ¥2,955.00

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94232ES60 100 μg ¥2,955.00 现货 Enquire

94232ES76 500 μg ¥9,885.00 现货 Enquire

94232ES25 25 μg ¥985.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No94232ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293表达系统

应用方法 ELISA用于检测PGF与抗体的结合活性 受体结合研究用于研究PGF与VEGFR1的相互作用

适用物种种属人

检测方法 SDS-PAGE HPLC LAL法 ELISA

生物特性参数 蛋白类型生长因子 种属来源人 生物活性描述ELISA数据：Immobilized Human PGF hFc Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-PGF Antibody hFc Tag with the EC50 of 34.1ng/ml determined by ELISA. Immobilized Human PGF hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human VEGF R1 His Tag with the EC50 of 12.8ng/ml determined by ELISA. 应用ELISA检测抗体结合；受体结合研究

技术参数纯度>95% (SDS-PAGE和HPLC) 内毒素水平<1.0 EU/μg (LAL法) 分子量预测48.6 kDa；糖基化后SDS-PAGE显示60-70 kDa 制剂形式冻干粉，溶剂为PBS (pH 7.4) 经0.22μm过滤，冻干前加入8%海藻糖作为保护剂 活性ELISA EC50：34.1 ng/ml（抗PGF抗体），12.8 ng/ml（VEGFR1） 储存条件-25~-15℃保存，有效期1年；复溶后在-85~-65℃无菌保存，有效期3个月 表达序列Leu19-Arg221 表达系统HEK293 标签C-hFc 外观冻干粉 复溶方法开盖前离心，使用蒸馏水复溶至浓度超过100 μg/mL，分装避免反复冻融 蛋白登录号P49763-1 产品别称PGFL PGF PlGF D12S1900