Recombinant Mouse PCSK9 Protein,His Tag

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HEK293细胞表达系统抗体药物靶点靶点蛋白研究

Product no.94577ES Sizes3 options SourceYeasen · Official

Cat. No. 94577ES60

现货 $3,585.00

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94577ES60 100 μg $3,585.00 现货 Enquire

94577ES76 500 μg $12,355.00 现货 Enquire

94577ES25 25 μg $1,195.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No94577ES

Specification高纯度、高活性、低内毒素、高批间一致性

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 抗体药物靶点作为PCSK9抗体药物靶点蛋白，用于药物研发与筛选 靶点蛋白研究用于研究PCSK9与LDLR等配体的相互作用

适用物种种属小鼠

检测方法 SDS-PAGE HPLC SPR LAL法 Tris-Bis PAGE

生物特性参数 蛋白类型重组PCSK9蛋白（前蛋白转化酶枯草溶菌素9） 种属来源Mouse（小鼠） 生物活性描述SPR Data: Human LDLR His Tag immobilized on CM5 Chip can bind Mouse PCSK9 His Tag with an affinity constant of 0.61 nM as determined in SPR assay. 应用靶点蛋白/抗体药物靶点蛋白

样本类型 重组蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Due to autocatalytic cleavage protein releases pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation protein migrates to 65-68 kDa (pro-form) and 15 kDa (mature form) based on Tris-Bis PAGE. 表达序列Gln35-Gln694 制剂形式Lyophilized from 0.22μm filtered solution in 20mM Tris 150mM NaCl 200mM L-Arginine (pH 8.2). Normally 8% trehalose is added as protectant before lyophilization. 活性SPR assay: Human LDLR His Tag immobilized on CM5 Chip can bind Mouse PCSK9 His Tag with an affinity constant of 0.61 nM. 储存条件-25 ~ -15℃保存，有效期1年；复溶后，无菌条件下-85 ~ -65℃保存，3个月有效期. 表达系统HEK293 标签C-His UniProt编号Q80W65 外观Lyophilized Powder 复溶浓度>100 μg/mL 复溶方法开盖前先离心；使用蒸馏水复溶冻干蛋白质，复溶浓度超过100 μg/mL；建议首次使用时将蛋白分装为每次用量，避免反复冻融. 产品别称PC9 HCHOLA3 FH3 NARC-1