Recombinant Human NKG2D/CD314 Protein,hFc-Flag Tag

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HEK293细胞表达系统抗体药物靶点蛋白

Product no.95935ES Sizes3 options SourceYeasen · Official

Cat. No. 95935ES25

现货 $1,245.00

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Cat.No Spec Price Status

95935ES25 25 μg $1,245.00 现货 Enquire

95935ES60 100 μg $3,495.00 现货 Enquire

95935ES76 500 μg $12,235.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No95935ES

Purity> 95% as determined by SDS-PAGE and HPLC.

Specification25 μg

AppearanceLyophilized Powder

Storage-25 ~ -15℃保存，收到货之后有效期1年。复溶后，无菌条件下，-85 ~ -65℃保存，3个月有效期。

Shelf Life1年

Origin翌圣生物科技(上海)股份有限公司

技术平台 HEK293细胞表达系统

应用方法 抗体药物靶点蛋白用于抗体药物开发及靶点验证

适用物种种属人

检测方法 SDS-PAGE HPLC LAL法 ELISA

生物特性参数 蛋白类型重组蛋白 种属来源人 生物活性描述【Blocking Data】Serial dilutions of Anti-NKG2D Antibody hFc Tag were added into Biotinylated Human ULBP-2 His TagHuman NKG2D hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.【ELISA Data】 Immobilized Human MICA His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D hFc Tag with the EC50 of 0.81μg/ml determined by ELISA. 应用抗体药物靶点蛋白

技术参数纯度> 95% as determined by SDS-PAGE and HPLC. 内毒素水平< 1.0 EU per μg by the LAL method. 分子量Predicted MW 43.4 kDa; migrates to 50-70 kDa due to glycosylation. 制剂形式Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 活性Blocking IC50=45.2 ng/ml; ELISA EC50=0.81 μg/ml. 储存条件-25 ~ -15℃保存，有效期1年；复溶后，-85 ~ -65℃保存3个月。 表达序列Phe78-Val216 表达系统HEK293 标签N-hFc-Flag 外观Lyophilized Powder 复溶方法开盖之前，先离心。使用蒸馏水复溶冻干的蛋白质，复溶浓度超过100 μg/mL。建议首次使用时将蛋白分装为每次实验用量，避免反复冻融。 AccessionP26718 产品别称CD314 D12S2489E KLR NKG2-D NKG2D