Trypsin 1:250 (powder) 胰蛋白酶1:250（粉末）

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细胞培养

Product no.40101ES Sizes1 options SourceYeasen · Official

Cat. No. 40101ES25 现货 ¥189.00

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40101ES25 25 g ¥189.00 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No40101ES

Specification中文别名（Chinese synonym）胰蛋白酶，猪胰脏来源；英文别名（English synonym）Trypsin, porcine pancreas; Parenzyme; Tryptar; Trypure; Parenzymol;

Origin翌圣生物科技(上海)股份有限公司

应用方法 细胞培养用于细胞传代，制备单细胞悬液；也用于初生组织解离

适用物种种属猪

生物特性参数来源猪胰腺 酶类型丝氨酸蛋白酶 切割位点赖氨酸或精氨酸的羧基端（脯氨酸除外） 亚基组成α亚基（2条多肽链）和β亚基（1条多肽链） 抑制剂有机磷化合物、天然胰蛋白酶抑制剂、银离子、AEBSF、抗蛋白酶、抑肽酶、DFP、亮抑肽酶、PMSF、TLCK等 酶原形式以无活性的胰蛋白酶原形式分泌于胰腺中

技术参数 工作浓度0.25-5% (w/v) 酶活≥250 U/mg 储存条件冻干粉2~8℃干燥保存，有效期2年 CAS号9002-07-7 分子量23.8 kDa 外观白色至类白色粉末 消光系数E1%280=14.3 等电点pH 10.5 溶解性溶于水，几乎不溶于乙醇和甘油 产品形式冻干粉 病毒灭活方式γ射线处理灭活病毒

实验类型 细胞分离与消化利用胰蛋白酶水解蛋白，将贴壁细胞消化下来或解离组织

Documents & Citations

Published citations

[1] Xu W, Che Y, Zhang Q, et al. Apaf-1 Pyroptosome Senses Mitochondrial Permeability Transition. Cell Metab. 2021;33(2):424-436.e10. doi:10.1016/j.cmet.2020.11.018(IF:21.567)

[2] Zhang W, Liu J, Li X, et al. Precise Chemodynamic Therapy of Cancer by Trifunctional Bacterium-Based Nanozymes. ACS Nano. 2021;15(12):19321-19333. doi:10.1021/acsnano.1c05605(IF:15.881)

[3] Peng J, Chen J, Xie F, et al. Herceptin-conjugated paclitaxel loaded PCL-PEG worm-like nanocrystal micelles for the combinatorial treatment of HER2-positive breast cancer. Biomaterials. 2019;222:119420. doi:10.1016/j.biomaterials.2019.119420(IF:12.479)