Hieff® Taq DNA Polymerase DNA聚合酶

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基因型鉴定菌落PCR

Product no.10101ES Sizes2 options Documents1 files SourceYeasen · Official

Cat. No. 10101ES80

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10101ES80 1000 U $128.00促~~$155.00~~ 现货 Enquire

10101ES92 10000 U $1,188.00促~~$1,245.00~~ 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No10101ES

Specification1、活性定义：用活性化的大马哈鱼精子DNA作为模板/引物，74℃，30 min内，摄入10 nmol的全核苷酸为酸性不溶物的活性定义为1个活性单位（U）。2、适用于基因型鉴定、菌落PCR等常规PCR。

Origin翌圣生物科技(上海)股份有限公司

应用方法 基因型鉴定常规PCR检测 菌落PCR菌落筛选

生物特性参数 试剂类型DNA聚合酶 应用基因型鉴定、菌落PCR等常规PCR 酶学活性5'→3'聚合酶活性和5'→3'外切酶活性，无3'→5'外切酶活性 产物特征扩增产物具有3'-dA，可直接用于TA克隆

技术参数 储存条件-25~-15℃保存，有效期2年 分子量94 KD 活性定义用活性化的大马哈鱼精子DNA作为模板/引物，74℃，30 min内，摄入10 nmol的全核苷酸为酸性不溶物的活性定义为1个活性单位（U）

实验类型 PCRDNA扩增

Documents & Citations

分子诊断原料 Open document

Published citations

[1] Lu Z, Yang S, Yuan X, et al. CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression in Bacillus subtilis. Nucleic Acids Res. 2019;47(7):e40. doi:10.1093/nar/gkz072(IF:11.147)

[2] Chen X, Liang H, Xi Z, et al. BM-MSC Transplantation Alleviates Intracerebral Hemorrhage-Induced Brain Injury, Promotes Astrocytes Vimentin Expression, and Enhances Astrocytes Antioxidation via the Cx43/Nrf2/HO-1 Axis. Front Cell Dev Biol. 2020;8:302. Published 2020 May 8. doi:10.3389/fcell.2020.00302(IF:5.186)

[3] Fu C, Xu J, Jia Z, Yao K, Chen X. Cataract-causing mutations L45P and Y46D promote γC-crystallin aggregation by disturbing hydrogen bonds network in the second Greek key motif. Int J Biol Macromol. 2021;167:470-478. doi:10.1016/j.ijbiomac.2020.11.158(IF:5.162)

[4] Chen X, Xu CX, Liang H, et al. Bone marrow mesenchymal stem cells transplantation alleviates brain injury after intracerebral hemorrhage in mice through the Hippo signaling pathway. Aging (Albany NY). 2020;12(7):6306-6323. doi:10.18632/aging.103025(IF:4.831)

[5] Fu C, Xu J, Yang X, Chen X, Yao K. Cataract-causing mutations L45P and Y46D impair the thermal stability of γC-crystallin. Biochem Biophys Res Commun. 2021;539:70-76. doi:10.1016/j.bbrc.2020.12.096(IF:3.575)

[6] Fei ZY, Wang WS, Li SF, et al. High expression of the TEFM gene predicts poor prognosis in hepatocellular carcinoma. J Gastrointest Oncol. 2020;11(6):1291-1304. doi:10.21037/jgo-20-120(IF:2.536)

[7] Zhang L, Li Y, Bao H, et al. Population structure and antimicrobial profile of Staphylococcus aureus strains associated with bovine mastitis in China. Microb Pathog. 2016;97:103-109. doi:10.1016/j.micpath.2016.06.005(IF:1.888)