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产品资料 PD-11300ES
第五代耐热逆转录酶 新逆转录酶 第五代耐热逆转录酶 新逆转录酶
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第五代耐热逆转录酶 新逆转录酶

Hifair® V Reverse Transcriptase 第五代耐热逆转录酶

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RT-PCRRT-qPCR低拷贝基因检测
产品编号11300ES 可选规格3 种 配套文档2 份 来源翌圣生物 · 官网
产品编号 11300ES92
现货 ¥1,538.00 促¥1,623.00
数量 1
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货号 规格 价格 状态
11300ES92 10000 U ¥1,538.00促¥1,623.00 现货 询价
11300ES93 5× 10000 U ¥5,338.00促¥5,623.00 现货 询价
11300ES98 200000 U ¥17,709.00促¥19,680.00 现货 询价
提交询价 价格为协议价,提交后由业务确认;大包装可申请试用装。

产品概述

技术参数

品牌翌圣生物 Yeasen
货号 / 型号11300ES
规格耐热性能优异。适用于RT-PCR/RT-qPCR。可提供无甘油产品(11301ES)形式,满足不同需求。
产地翌圣生物科技(上海)股份有限公司
技术平台 RT-PCR RT-qPCR
应用方法 RT-PCRRNA检测和扩增 RT-qPCR基因表达定量分析 低拷贝基因检测适用于少量模板及低拷贝基因的逆转录
检测方法 qPCR
生物特性参数 试剂类型逆转录酶/工具酶 应用RT-PCR/RT-qPCR,低拷贝基因检测 模板类型RNA 产物类型cDNA
样本类型 RNA
技术参数 储存条件-25~-15℃保存,有效期2年 热稳定性可耐受高达60℃的反应温度,58℃孵育30min仍能保持70%活性 最大cDNA合成长度10 kb 反应温度最高60℃
实验类型 逆转录将RNA模板反转录为cDNA,用于后续PCR或qPCR检测 cDNA合成合成全长cDNA,用于克隆和表达分析

文档与文献

已发表文献

1. Liu C X, Li X, Nan F, et al. Structure and degradation of circular RNAs regulate PKR activation in innate immunity[J]. Cell, 2019, 177(4): 865-880. e21.(IF31.398)

2. Fan H, Hong B, Luo Y, et al. The effect of whey protein on viral infection and replication of SARS-CoV-2 and pangolin coronavirus in vitro[J]. Signal transduction and targeted therapy, 2020, 5(1): 1-3.(IF13.493)

3. Zhou L, Hou B, Wang D, et al. Engineering Polymeric Prodrug Nanoplatform for Vaccinatio Immunotherapy of Cancer[J]. Nano Letters, 2020.(IF12.279)

4. Wang J., et al., The mycobacterial phosphatase PtpA regulates the expression of host genes and promotes cell proliferation[J]. Nat Commun. 2017 Aug 15;8(1):244.(IF 12.353)

5. Tao L, Yi Y, Chen Y, et al. RIP1 kinase activity promotes steatohepatitis through mediating cell death and inflammation in macrophages[J]. bioRxiv, 2020.( IF10.717)

6. Ma D, Zhao Y, Huang L, et al. A novel hydrogel-based treatment for complete transection spinal cord injury repair is driven by microglia/macrophages repopulation[J]. Biomaterials, 2020, 237: 119830.(IF10.317)

7. Lin Z, Xia S, Liang Y, et al. LXR activation potentiates sorafenib sensitivity in HCC by activating microRNA-378a transcription[J]. Theranostics, 2020, 10(19): 8834. (IF8.579) 11120ES

8. Liu D, Nie W, Li D, et al. 3D printed PCL/SrHA scaffold for enhanced bone regeneration[J]. Chemical Engineering Journal, 2019, 362: 269-279.(IF8.355)

9. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide PtrCLE20 inhibits vascular cambium activity in Populus[J]. Plant Biotechnology Journal, 2020, 18(1): 195-206.(IF8.154)

10. Chen L, Lam J C W, Tang L, et al. Probiotic modulation of lipid metabolism disorders caused by perfluorobutanesulfonate pollution in zebrafish[J]. Environmental Science & Technology, 2020.(IF7.864)

11. Zhao P, Zhang J, Wu A, et al. Biomimetic codelivery overcomes osimertinib-resistant NSCLC and brain metastasis via macrophage-mediated innate immunity[J]. Journal of Controlled Release, 2020. ( IF7.727)

12. Xu X, Gao J, Dai W, et al. Gene activation by a CRISPR-assisted trans enhancer[J]. eLife, 2019, 8: e45973.(IF7.551)

13. Zhao Y, Wang H P, Yu C, et al. Integration of physiological and metabolomic profiles to elucidate the regulatory mechanisms underlying the stimulatory effect of melatonin on astaxanthin and lipids coproduction in Haematococcus pluvialis under inductive stress conditions[J]. Bioresource Technology, 2020, 319: 124150.(IF7.539)

14. Li X, Zhang X, Zhao Y, et al. Cross-talk between gama-aminobutyric acid and calcium ion regulates lipid biosynthesis in Monoraphidium sp. QLY-1 in response to combined treatment of fulvic acid and salinity stress[J]. Bioresource Technology, 2020, 315: 123833.(IF7.539)

15. Nie, W., et al., Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering[J]. Carbon, 2017.116, 325-337.(IF 7.082)

16. Zhu Y, Song D, Zhang R, et al. A xylem‐produced peptide Ptr CLE 20 inhibits vascular cambium activity in Populus[J]. Plant biotechnology journal, 2019.(IF6.84)

17. Peng L, Wang Y, Yang B, et al. Polychlorinated biphenyl quinone regulates MLKL phosphorylation that stimulates exosome biogenesis and secretion via a short negative feedback loop[J]. Environmental Pollution, 2020: 115606.(IF6.793)

18. Li A, Liu Q, Li Q, et al. Berberine reduces pyruvate-driven hepatic glucose production by limiting mitochondrial import of pyruvate through mitochondrial pyruvate carrier 1[J]. EBioMedicine, 2018, 34: 243-255. (IF6.68)

19. Lou M D, Li J, Cheng Y, et al. CREB mediates glucagon action to upregulate hepatic MPC1: inhibitory effect of ginsenoside Rb1 on hepatic gluconeogenesis[J]. British journal of pharmacology, 2019.(IF6.583)

20. Fan L, Ye H, Wan Y, et al. Adaptor protein APPL1 coordinates HDAC3 to modulate brown adipose tissue thermogenesis in mice[J]. Metabolism, 2019, 100: 153955.(IF6.513)

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