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Product data sheet PD-15222ES
XmnI (10 U/μL) XmnI (10 U/μL)
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XmnI (10 U/μL)

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分子克隆
Product no.15222ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15222ES76 现货 $158.00 促$165.00
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Cat.No Spec Price Status
15222ES76 500 U $158.00促$165.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15222ES
Specification500 U
Origin翌圣生物科技(上海)股份有限公司
应用方法 分子克隆用于DNA片段制备及双酶切等
适用物种种属 通用
生物特性参数 试剂类型限制性内切酶 应用分子克隆/双酶切 来源木薯萎蔫病黄单胞菌(重组表达于大肠杆菌) 酶类型Type IIP型限制酶 识别序列5'-GAANN↓NNTTC-3' / 3'-CTTNN↑NNAAG-5' 切割末端平末端 反应条件1×Cut Buffer C缓冲液;37°C孵育 失活条件80℃温育20 min 酶活定义1活性单位(U)是指在50 μL反应体系中,37℃ 1h内完全酶切1μg λDNA所需的酶量 甲基化修饰影响CpG:剪切受影响;EcoBI:剪切受阻 缓冲液兼容性兼容FuniCut® Buffer 星号活性避免长时间酶切以防止出现星号活性
技术参数 浓度10 U/μL 储存条件-25~-15℃保存,有效期2年
实验类型 限制性酶切识别并切割GAANN/NNTTC序列,产生平末端

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