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Product data sheet PD-15215ES
Sgel (5 U/μL) Sgel (5 U/μL)
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Sgel (5 U/μL)

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分子克隆
Product no.15215ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15215ES50 现货 $408.00 促$425.00
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Cat.No Spec Price Status
15215ES50 250 U $408.00促$425.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15215ES
Specification250 U
Origin翌圣生物科技(上海)股份有限公司
应用方法 分子克隆DNA片段操作
生物特性参数 试剂类型限制性内切酶 应用分子克隆 同裂酶Bse3DI BseMI 甲基化修饰影响Dcm: 总是切割被Dcm甲基转移酶甲基化的DNA;CpG: 切割与CpG甲基化序列重叠的靶点
技术参数 浓度5 U/μL 缓冲液组分Storage Buffer中含预混合BSA 储存条件-25~-15℃保存,有效期2年 反应条件1×Sgel Buffer;37℃孵育 失活条件80℃温育20 min 识别位点5'-ᵐ⁵CNNG(N)9↓-3' 3'-GNNC(N)13↑-5' 酶活定义在50 μl反应体系中,加入1 μg pUC19-SgeI DNA (Dcm+)底物,在37℃孵育1 h,不断增加酶量,直至酶切产物DNA带型不随着酶量的增加而发生变化
实验类型 限制性酶切切割DNA靶标

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