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Product data sheet PD-15023ES
FuniCut® SacI FuniCut® SacI
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FuniCut® SacI

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分子克隆DNA切割
Product no.15023ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15023ES60 现货 ¥158.00 促¥165.00
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15023ES60 100 T ¥158.00促¥165.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15023ES
Specification识别位置5'-GAGCT↓C-3'3'-C↑TCGAG-5'推荐反应条件1×FuniCut® 缓冲液;最适37℃孵育酶活20 U/μL失活条件80°C孵育20 min同裂酶Psp124BI, SstI甲基化修饰影响Dam、Dcm、CpG、E
Origin翌圣生物科技(上海)股份有限公司
应用方法 分子克隆用于DNA片段的切割与克隆 DNA切割用于快速切割DNA
生物特性参数 试剂类型限制性内切酶 应用快速切割质粒DNA、PCR产物、基因组DNA等 识别序列5'-GAGCT↓C-3' / 3'-C↑TCGAG-5' 同裂酶Psp124BI SstI 甲基化修饰影响Dam、Dcm、CpG、EcoKI、EcoBI:无影响
技术参数 浓度20 U/μL 缓冲液组分1×FuniCut® 缓冲液 储存条件-25~-15℃保存,有效期2年 失活条件80°C孵育20 min 最适反应温度37℃
实验类型 限制性内切酶酶切用于快速切割质粒DNA、PCR产物、基因组DNA等DNA底物

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