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Product data sheet PD-15063ES
FuniCut® HpaII FuniCut® HpaII
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FuniCut® HpaII

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通用分子克隆质粒线性化
Product no.15063ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15063ES70 现货 ¥158.00 促¥165.00
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15063ES70 200 T ¥158.00促¥165.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15063ES
Specification识别位置5'-C↓CGG-3'3'-GGC↑C-5'推荐反应条件1×FuniCut® 缓冲液;最适37℃孵育酶活20 U/μL失活条件80℃孵育20 min同裂酶MspI, HapII, BsiSI甲基化修饰影响CpG:剪切受阻
Origin翌圣生物科技(上海)股份有限公司
技术平台 通用
应用方法 分子克隆载体构建 质粒线性化制备线性化DNA
生物特性参数 试剂类型限制性内切酶 应用质粒线性化、载体构建
样本类型 质粒DNA PCR产物 基因组DNA
技术参数 浓度20 U/μL 缓冲液组分1×FuniCut® 缓冲液 储存条件-25~-15℃保存,有效期2年 识别位点5'-C↓CGG-3' / 3'-GGC↑C-5' 推荐反应条件1×FuniCut® 缓冲液;最适37℃孵育 失活条件80℃孵育20 min 同裂酶MspI HapII BsiSI 甲基化修饰影响CpG:剪切受阻 反应时间5~15 min 有效期2年
实验类型 限制性内切酶酶切快速切割DNA

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