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Product data sheet PD-15074ES
FuniCut® DraIII FuniCut® DraIII
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FuniCut® DraIII

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限制性内切酶分子克隆载体构建
Product no.15074ES Sizes1 options Documents1 files SourceYeasen · Official
Cat. No. 15074ES30 现货 $158.00 促$165.00
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15074ES30 30 T $158.00促$165.00 现货 Enquire
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Overview

Specifications

Brand翌圣生物 Yeasen
Cat.No15074ES
Specification识别位置5'-CAC(N)3↓GTG-3'3'-GTG↑(N)3CAC-5'推荐反应条件1×FuniCut® 缓冲液;37°C孵育酶活20 U/μL失活条件不可热失活,请使用酚氯仿抽提或柱纯化同裂酶AdeI甲基化修饰影响CpG、EcoKI:
Origin翌圣生物科技(上海)股份有限公司
技术平台 限制性内切酶
应用方法 分子克隆载体构建 载体构建DNA片段拼接
生物特性参数 试剂类型限制性内切酶 应用分子克隆;载体构建 甲基化修饰敏感性CpG、EcoKI:剪切受影响;EcoBI:剪切受阻;Dam、Dcm:无影响
样本类型 质粒DNA PCR产物 基因组DNA
技术参数 浓度20 U/μL 缓冲液组分1×FuniCut® 缓冲液 储存条件-25~-15℃保存,有效期2年 识别位点5'-CAC(N)3↓GTG-3' 3'-GTG↑(N)3CAC-5' 推荐反应条件1×FuniCut® 缓冲液;37°C孵育 失活条件不可热失活,请使用酚氯仿抽提或柱纯化 同裂酶AdeI 反应时间5-15 min
实验类型 限制性酶切切割DNA

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