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ApplicationsPlasmid Agarose HP-质粒纯化好帮手
📂 蛋白纯化 Type ID 5003
Plasmid Agarose HP-质粒纯化好帮手
Source: Yeasen Biotech | Original: https://www.yeasen.com/solutiondetail/714 ↗
质粒DNA应用
质粒是闭合环状的双链超螺旋DNA分子,是目前基因治疗领域及疫苗制备中最重要的工具之一。质粒DNA可以作为疫苗或治疗性药物单独使用,也可以在病毒载体生产中作为病毒包装的原料,在mRNA疫苗或药物的生产中,可以作为体外转录反应的模板,用于mRNA的体外转录制备。

质粒纯化工艺

产品简介
Plasmid Agarose HP超螺旋DNA纯化介质是一种亲硫芳香族吸附层析填料,其选择性可将质粒 DNA的超螺旋共价闭环结构与开环结构分离。
表1:Plasmid Agarose HP产品性质
基质 | 高度交联的琼脂糖凝胶 |
配基 | 巯基吡啶 |
粒径 | 36-44 μm |
载量 | >3 mg/mL基质 |
耐压 | 0.3 MPa |
最大流速 | 220 cm/h |
PH稳定性 | 3~13(长期),2~14(CIP,短期) |
化学稳定性 | 在以下液体中稳定:所有常用的水相缓冲液;1mol/L 氢氧化钠;70% 乙醇;40%异丙醇;1M 醋酸 |
储存缓冲液 | 20%乙醇,4~30℃ |
产品特点

使用方法

平衡液:2.0M(NH4)2SO4,10mM EDTA,100mM Tris-HCl,pH 7.5
洗脱液:0.3M NaCl,1.7M(NH4)2SO4,10mM EDTA,100mM Tris-HCl,pH 7.5
注意:不同序列及大小的质粒,纯化时缓冲液及载量要适当的调整。
测试结果

图1 Plasmid Agarose HP介质纯化质粒色谱图

图2 Plasmid Agarose HP介质纯化质粒电泳图
产品订购
产品名称 | 产品编号 | 规格 |
20500ES25 | 25 mL | |
20500ES60 | 100 mL | |
20500ES76 | 500 mL | |
20500ES03 | 1 L |
Source
Source:
Yeasen Biotech
Original URL:
https://www.yeasen.com/solutiondetail/714
Source ID: 714
Content is compiled from Yeasen Biotech public materials; all rights belong to the original author.
Products CoveredFrom source article
IMG
Protein L 4FF Chromatography Column, 1 m...
Not listed yet IMG rProtein L Agarose Resin 4FF 蛋白L琼脂糖快速纯化树...
Not listed yet IMG Protein G 4FF Chromatography Column, 1 m...
Not listed yet IMG Protein G 4FF Chromatography Column, 5 m...
Not listed yet IMG SuRi rProtein A Agarose Resin 耐碱重组蛋白A琼脂糖...
Not listed yet IMG rProtein G Agarose Resin 蛋白G琼脂糖纯化树脂
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Not listed yet IMG rProtein L Agarose Resin 4FF 蛋白L琼脂糖快速纯化树...
Not listed yet IMG Protein G 4FF Chromatography Column, 1 m...
Not listed yet IMG Protein G 4FF Chromatography Column, 5 m...
Not listed yet IMG SuRi rProtein A Agarose Resin 耐碱重组蛋白A琼脂糖...
Not listed yet IMG rProtein G Agarose Resin 蛋白G琼脂糖纯化树脂
Not listed yet IMG Protein A 4FF Chromatography Column, 1 m...
Not listed yet IMG rProtein A/G Agarose Resin 蛋白A/G琼脂糖纯化树脂
Not listed yet IMG BiotSep Streptavidin 6FF Chromatography ...
Not listed yet IMG BiotSep Streptavidin 6FF Chromatography ...
Not listed yet IMG Endotoxin Removal Agarose Resin, High Pe...
Not listed yet IMG Anti-DYKDDDDK (Flag) Affinity Gel (Secre...
Not listed yet IMG Anti-Myc Affinity Gel Anti-Myc标签亲和纯化凝胶
Not listed yet IMG Anti-HA Affinity Gel Anit-HA亲和纯化凝胶
Not listed yet IMG Anti-His Affinity Gel Anti-His标签亲和纯化凝胶
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