LiteratureNature Chemical Biology| 翌圣转染试剂助力大麻素受体CB1的变构调节机制研究
文末福利 | 转染试剂荣登《Nature Chemical Biology》,助力大麻素受体CB1的变构调节机制研究
大麻素受体CB1是人类中枢神经系统表达量最高的G蛋白偶联受体(GPCR)之一,主要位于脑、脊髓与外周神经系统中,参与调节人体的新陈代谢、食欲、疼痛、记忆和学习、神经系统和心血管系统等生理过程。其配体(包括激动剂和拮抗剂)可用于治疗疼痛、炎症、神经退行性疾病、肥胖、药物成瘾以及肝纤维化等。靶向CB1的药物开发备受关注,众多药企与科研团队都聚焦于该受体的药物研发。但CB1变构调节的具体分子机制尚不清楚,严重阻碍了变构药物的研发。

2022年5月30日,四川大学华西医院生物治疗国家重点实验室杨胜勇教授团队联合生物治疗国家重点实验室/肾脏内科邵振华研究员团队在《Nature Chemical Biology》期刊(IF:15.04)在线发表了题为《Molecular mechanism of allosteric modulation for the cannabinoid receptor CB1》的研究论文。充分运用计算机辅助药物设计的多种技术方法对大麻素受体CB1分别与正向变构调节剂ZCZ011及负向别构调节剂ORG27569的相互作用机理进行研究,让我们对CB1受体别构调节的分子机理有了更充分的理解,为设计与筛选更合适的药物提供了重要的理论依据。

在该研究中,研究团队选择了翌圣的转染试剂PEI40000用于相关蛋白的研究:

目前转染试剂系列的产品已经荣登《Nature》、《Cell》等多个顶级期刊,获得科研大牛们认可!
以下仅展示部分论文:
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翌圣生物转染试剂产品
货号 | |||||||||||
产品 名称 | |||||||||||
细胞 类型 | 常规细胞 | 常规细胞 | 常规细胞 | 常规细胞 | 昆虫细胞 | 常规细胞 | 常规细胞 | 常规细胞 | 常规细胞 | 常规细胞 | 常规细胞 |
难转染 | 难转染 | 难转染 | |||||||||
原代 | 原代 | 原代 | |||||||||
干细胞 | 干细胞 | 干细胞 | |||||||||
核酸 类型 | DNA | DNA | DNA | DNA | DNA | DNA | DNA | DNA | DNA | ||
siRNA | siRNA | ||||||||||
miRNA | miRNA | ||||||||||
ASO | ASO | ||||||||||
mRNA | mRNA | ||||||||||
应用 场景 | 用于各种核酸及原代等难转染细胞系 | 普遍用于多种细胞DNA转染 | 用于siRNA和miRNA转染,在多种难转染细胞上均能高表达 | mRNA转染专用,在多种难转染细胞上均能高表达 | 针对sf9和High Five等昆虫细胞进行杆状病毒及质粒DNA转染 | 通用型病毒包装转染试剂(适用于LV及AAV) | AAV病毒包装专用转染试剂 | LV慢病毒包装专用转染试剂 | CHO悬浮细胞瞬转表达蛋白、抗体,含转染试剂、培养基、补料 | 293悬浮细胞瞬转表达蛋白、抗体,含转染试剂、培养基、补料 | 用于抗体、蛋白、细胞因子等瞬转表达,满足CHO及293悬浮细胞系需求 |
对标 竞品 | thermo-lipo3000 | thermo-lipo2000 | thermo-RNAiMAX | thermo-Messenger MAX | thermo-Cellfectin | polyplus-PEIpro GMP | polyplus-FectorVIR-AAV GMP/ PEIpro | polyplus-FectorVIR-LV/PEIpro | thermo-ExpiCHO 瞬转 试剂盒 | thermo-Expi293 瞬转 试剂盒 | polyplus-FectorPRO |
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