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ApplicationsDSS诱导小鼠AOM/DSS结直肠癌模型的建立

📂 建模实验 Type ID 5003

👁 19,341 views 🗓 2026-10-12 Source ID：962

AOM/DSS模型是目前使用最广泛的UC化学诱导模型，临床症状学、形态学及病理学观察证明，该模型与人类溃疡性结肠炎癌变过程极为相似。AOM（60751ES）引起鸟嘌呤O6甲基化是诱导基因突变损伤最主要的原因，基因突变使细胞过度增殖，形成特异性的大肠肿瘤。DSS的致炎机制尚未阐明，可能与DSS的负电荷影响 DNA 合成、抑制上皮细胞增生、破坏肠黏膜屏障、导致巨

Source: Yeasen Biotech ｜ Original: https://www.yeasen.com/solutiondetail/962 ↗

图1.来源pixabay

作用机制

偶氮甲烷（AOM）能诱导DNA产生O6-甲基鸟嘌加合物，导致G→A转化。

实验动物

BALB/c小鼠（7周龄，雄性）

实验材料

DSS(Yeasen#60316ES,MW36000-50000)、AOM

实验前准备

DSS配制：使用无菌水配制浓度1-3% DSS溶液，储存在4℃备用。

AOM配制：用无菌水溶解AOM配制10 mg/mL的储存液，分装后放到-20℃保存，避免反复冻融。使用之前，融化分装母液并用无菌生理盐水1:10倍稀释。

实验步骤

1.第1天称重并标记小鼠，实验组小鼠腹腔注射偶氮甲烷 AOM（10 mg/kg）；
2.用常规饮用水喂食一周；
3.用2.5%浓度DSS替换饮用水喂食一周；
4.用常规饮用水喂食2周；
5.步骤3和步骤4重复3次。

图2.AOM/DSS结直肠癌造模步骤

结果分析

图3.(A)AOM/DSS 诱导的结直肠癌示意图， (B)小鼠体重、(C)腹泻评分、(D)出血评分和(E) 代表性全结肠和结肠癌数的变化。

图4.结肠切片HE染色结果

参考文献

[1] Jia-Rong Huang, Sheng-Te Wang,Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.11:586885.(IF=5.988,DSS yeasen 60316ES)

翌圣DSS产品优势

翌圣生物提供高品质DSS（Cat.NO：60316ES，MW:36000~50000），具有以下优势：

1. 高纯度（＞98%），硫含量17-19%，游离硫＜0.2%，大量数据文献支持，广泛应用于UC模型的构建。

2. 多种属动物中均可造模：小鼠、大鼠、斑马鱼、猪、果蝇；

3. 症状表现与人UC极度相似：可用于研究急、慢性结肠炎的发生发展机制，也可用于药物的药效研究。

4. 简单易行，成模率高，重复性强

5. 联合氧化偶氮甲烷(azoxymethane, AOM)用药，可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型，成功模拟IBD诱发CAC的过程。

建模效率与进口产品一致，产品价格低至进口价格的1/3，大量现货库存。

产品订购

货号	产品名称	规格	应用场景
60316ES	ColitCare^®Dextran Sulfate Sodium Salt（DSS）结肠炎建模用葡聚糖硫酸钠盐 MW : 36000～50000	25 g/100 g/500 g/1000 g	常被用于诱导结肠炎模型。
60751ES	Azoxymethane 偶氮甲烷(AOM) \|CAS 25843-45-2	1 mg/5 mg/10 mg （液体：135 mM in Water）	与葡聚糖硫酸钠盐DSS 一起用于结直肠癌的小鼠模型创建。
60736ES	Low Density Lipoprotein Cholesterol Content Assay kit 低密度脂蛋白胆固醇（LDL-C）含量检测试剂盒（微量法）	96T	测定低密度脂蛋白胆固醇含量。
60737ES	High Density Lipoprotein Cholesterol Content Assay Kit 高密度脂蛋白胆固醇（HDL-C）含量检测试剂盒（微量法）	96T	测定高密度脂蛋白胆固醇含量。
61220ES	FITC-Dextran (MW 4000) FITC标记葡聚糖(MW 4000)	5 mg/10mg/50mg	评估肠的渗透性。
61223ES	FITC-Dextran (MW 40000) FITC标记葡聚糖(MW 40000)	5 mg/10mg/50mg	评估肠的渗透性。
18820ES	MolPure® Stool DNA Kit 粪便DNA提取试剂盒	5 T/50 T/200 T	能高效获得优质的粪便DNA，有利于后续对肠道微生物的研究。
60524ES	Hematoxylin and Eosin Staining Kit 苏木素伊红(H&E)染色试剂盒	2×100 mL	用于免疫组化中组织切片，血涂片，骨髓切片的染色。
60403ES	Urine Fecal Occult Blood Test Kit 尿粪隐血检测试剂盒(联苯胺法)	100T	对慢性消化道出血，如：消化性溃疡等的研究筛选均具有重要价值。
60534ES	AB-PAS染色液	6×50 mL/6×100 mL	可鉴别同一组织中的中性黏蛋白和酸性黏蛋白。
41403ES	PBS(1×)细胞培养级	500 mL	最常用的缓冲溶液。

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2022

[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717

[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143

[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451

[4] Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076

[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869

2021

[1] Li Zhao, Fei Wang , Zhengwei Cai，et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].Chemical Engineering Journal.424(2021)130464. IF=16.744

[2] Hao H, Zhang X, Tong L, Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561

[3] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717

[4] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943

[5] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

2020

[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561

[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

BEFORE 2020

[1] Oehlers SH, Flores MV, Hall CJ, Crosier KE, Crosier PS. Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis. Dis Model Mech. 2012 Jul;5(4):457-67.IF=4.973

[2] Kim CJ, Kovacs-Nolan JA, Yang C, Archbold T, Fan MZ, Mine Y. l-Tryptophan exhibits therapeutic function in a porcine model of dextran sodium sulfate (DSS)-induced colitis. J Nutr Biochem . 2010 Jun;21(6):468-75.IF=6.048

[3] Karpowicz , P., Perez, J. & Perrimon , N.,. The Hippo tumor suppressor pathway regulates intestinal stem cell regeneration. Development (Cambridge, England), 2010,137(24), pp.4135–4145.IF=6.868

[4] Fan H, Chen W, Zhu J, et al. Toosendanin alleviates dextran sulfate sodium-induced colitis by inhibiting M1 macrophage polarization and regulating NLRP3 inflflammasome and Nrf2/HO-1 signaling[J]. International immunopharmacology, 2019, 76: 105909.IF=3.943

往期精彩

1.不同分子量葡聚糖硫酸钠选购指南：高品质DSS葡聚糖硫酸钠盐

2.DSS结肠炎造模整体解决方案：Dextran Sulfate Sodium Salt（DSS）溃疡性结肠炎模型的建立 (yeasen.com)

Source

Source: Yeasen Biotech

Original URL: https://www.yeasen.com/solutiondetail/962

Source ID: 962

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