ApplicationsDSS诱导猪溃疡性结肠炎模型的建立
DSS诱导猪溃疡性结肠炎模型的建立
背景概述
肠道通透性增加和相关体重减轻是DSS结肠炎动物模型的常⻅特征。D-甘露醇是⼀种惰性小碳水化合物分⼦，可沿整个隐窝-绒毛轴吸收，⽤于评估体内肠道通透性。
图源百度
实验动物
4-5天大，约克郡小猪
实验材料
DSS（yeasen DSS 60316ES,MW36000-50000）
实验步骤
1. 以商业代乳粉配⽅饮⾷，每天喂食三次，直到实验结束；
2. 将动物分为阳性对照组（Pos），阴性对照组（Neg），实验组（Trp）;
3. 阳性对照组（Pos）：灌注DSS;阴性对照组（Neg）：灌注生理盐水;实验组（Trp）:灌注DSS+治疗药物；
4. 每只小猪每日摄入量1.25g DSS/kg，灌注5天。
实验结果
图1. 肠道通透性分析
DSS诱导致小猪体内D-甘露醇浓度升高，肠道屏障被破坏。
图2. HE病理染色切片（A为Neg组；B为Pos组）
DSS处理的Pos组隐窝结构扭曲、炎症细胞浸润到粘膜和粘膜下层、隐窝脓肿和隐窝炎。
图3. 炎症因子检测
DSS处理的Pos组中两种细胞因⼦的浓度均升高[1]。
翌圣DSS产品优势
翌圣提供高品质DSS（Cat.NO：60316ES，MW:36000~50000），具有以下优势：
1、高纯度（＞98%），硫含量17-19%，游离硫＜0.2%，大量数据文献支持，广泛应用于UC模型的构建。
2、多种属动物中均可造模：小鼠、大鼠、斑马鱼、猪、果蝇；
3、症状表现与人UC极度相似：可用于研究急、慢性结肠炎的发生发展机制，也可用于药物的药效研究。
4、简单易行，成模率高，重复性强
5、联合氧化偶氮甲烷(azoxymethane, AOM)用药，可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型，成功模拟IBD诱发CAC的过程。
建模效率与进口产品一致，产品价格低至进口价格的1/3，大量现货库存。
产品订购
产品名称 | 货号 | 规格 |
60316ES25 | 25 g | |
60316ES60 | 100 g | |
60316ES76 | 500 g | |
60316ES80 | 1 kg | |
点击产品名称查看详情
其他产品推荐
货号 | 产品名称 | 规格 | 应用场景 |
18820ES | 5 T/50 T/200 T | 能高效获得优质的粪便DNA，有利于后续对肠道微生物的研究。 | |
18528ES | MolPure® Mag48 Soil/Stool DNA Kit FA 磁珠法48孔土壤 /粪便DNA提取试剂盒FA（预封装） | 48 T | 磁珠法提取样本基因组DNA。 |
18526ES | 20 T/50 T/200 T | 磁珠法提取样本基因组DNA。 | |
60524ES | 2×100 mL | 用于免疫组化中组织切片，血涂片，骨髓切片的染色。 | |
60403ES | 100T | 对慢性消化道出血，如：消化性溃疡等的研究筛选均具有重要价值。 | |
60534ES | 6×50 mL/6×100 mL | 可鉴别同一组织中的中性黏蛋白和酸性黏蛋白。 | |
60751ES | 1 mg/5 mg/10 mg（液体：135 mM in Water） | 与葡聚糖硫酸钠盐DSS 一起用于结直肠癌的小鼠模型创建。 |
产品已发表文章(部分)
向下滑动查看
2022
[1] Lujuan Xing, Lijuan Fu, Songmin Cao,et al.The Anti-Inflammatory Effect of Bovine Bone-Gelatin-Derived Peptides in LPS-Induced RAW264.7 Macrophages Cells and Dextran Sulfate Sodium-Induced C57BL/6 Mice[J]. Nutrients 2022, 14, 1479. IF=5.717
[2] Lei-NingChen,TaoJing,Zi-BinLin,et al.Metabolomic and transcriptomic responses of mouse testis to the dextran sulfate sodium induced colitis[J].Reproductive Toxicology.2022, Pages 35-42.IF=3.143
[3] YuangengLi,PingYu,WenwenFu,et al.Polysaccharides from Panax ginseng C. A. Meyer alleviated DSS-induced IBD by inhibiting JAK2/STAT1/NLPR3 inflammasome signalling pathway in mice[J].Journal of Functional Foods.2022, 105013. IF=4.451
[4] Wang S, Huang J, Tan KS, et al.Isosteviol Sodium Ameliorates Dextran Sodium Sulfate-Induced Chronic Colitis through the Regulation of Metabolic Profiling, Macrophage Polarization, and NF-B Pathway[J].Oxidative Medicine and Cellular Longevity. 2022,4636618. IF=5.076
[5] Chenglu Sun, Jingpeng Hao,Hong Qin,et al.Endometrial Regenerative Cell-Derived Conditioned Medium Alleviates Experimental Colitis.Stem Cells Int 2022;2022 IF=3.869
2021
[1] Li Zhao, Fei Wang,Zhengwei Cai,et al.Improving drug utilization platform with injectable mucoadhesive hydrogel for treating ulcerative colitis[J].chemical engineering journal.424(2021)130464.IF=16.744
[2] Hao H, Zhang X, Tong L, Liu Q,et al.Lactobacillus plantarumEffect of Extracellular Vesicles Derived From Q7 on Gut Microbiota and Ulcerative Colitis in Mice[J].Frontiers in Immunology.2021.777147 .IF=7.561
[3] Lingjun Tong, Xinyi Zhang , Haining Hao,et al.Lactobacillus rhamnosus GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice[J].Nutrients.2021, 13, 3319. IF=5.717
[4] Zhipeng Gu , Yujie Zhu, Fengfeng Mei,et al.Tilapia head glycolipids protect mice against dextran sulfate sodium-induced colitis by ameliorating the gut barrier and suppressing NF-kappa B signaling pathway[J].International Immunopharmacology.96 (2021) 107802. IF=3.943
[5] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423
[6] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561
2020
[1] Jia-Rong Huang, Sheng-Te Wang, Meng-Ning Wei,et al.Piperlongumine Alleviates Mouse Colitis and Colitis-Associated Colorectal Cancer[J].Frontiers in Pharmacology.2020.586885. IF=7.561
[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048
[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245
往期精彩
1. 不同分子量葡聚糖硫酸钠选购指南：高品质DSS葡聚糖硫酸钠盐
2. 小鼠造模方法及具体步骤：Dextran Sulfate Sodium Salt（DSS）结肠炎造模解决方案 (yeasen.com)
3. DSS结肠炎造模整体解决方案：Dextran Sulfate Sodium Salt（DSS） 溃疡性结肠炎模型的建立 (yeasen.com)
参考文献
[1] Connie J. Kim, et al. Journal of Nutritional Biochemistry 21 (2010) 468–475.
Not listed yet IMG Caerulein 雨蛙素
Not listed yet IMG ColitCare® Dextran Sulfate Sodium Salt（D...
Not listed yet IMG Rotenone 鱼藤酮（10 mM in DMSO）溶液
Not listed yet IMG Taurocholic acid Sodium Salt牛磺胆酸钠
Not listed yet IMG MPTP 盐酸盐
Not listed yet IMG Thioacetamide 硫代乙酰胺
Not listed yet IMG 7,12-二甲基苯并[a]蒽（DMBA）
Not listed yet IMG Uric acid sodium salt 尿酸钠
Not listed yet IMG Imiquimod 咪喹莫特
Not listed yet IMG 6-Hydroxydopamine Hydrobromide 6-羟基多巴胺合溴...
Not listed yet IMG MPTP 盐酸盐溶液（10 mM in DMSO）
Not listed yet IMG Rotenone 鱼藤酮（纯度≥99%）
Not listed yet IMG N-甲基-N'-硝基-N-亚硝基胍
Not listed yet IMG 4-Nitroquinoline N-oxide 4-硝基喹啉-N-氧化物
Not listed yet