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ApplicationsDSS诱导斑马鱼溃疡性结肠炎模型的建立

📂 建模实验 Type ID 5003

👁 10,532 views 🗓 2026-10-12 Source ID：964

DSS诱导斑马鱼溃疡性结肠炎模型的建立

Source: Yeasen Biotech ｜ Original: https://www.yeasen.com/solutiondetail/964 ↗

斑马鱼作为肠道损伤模型的应用已得到充分证实，葡聚糖硫酸钠（Dextran Sulfate Sodium Salt，DSS）诱导的斑马鱼IBD模型模拟了哺乳动物IBD表型的不同特征，包括肠道中性粒细胞炎症、过多粘液生成、肠淋巴管生成增加以及前炎性细胞因子上调。

图片来源：包图网

实验动物

3dpf（days post fertilization) 斑马鱼

实验材料

DSS（Yeasen 60316ES，MW:36000~50000）

实验步骤

1. 将斑马鱼胚胎培养在含甲基蓝的E3胚胎培养基中，28.5 ℃，培养至1 dpf；

2. 1dpf 后使用不含亚甲基蓝的E3胚胎培养基，培养至3 dpf；

3. 用E3培养基配制浓度为10% DSS的储存液;

4. 用培养基稀释DSS至最大非致死剂量（DSS浓度参考：0.5%）；

5. 用0.5%的DSS处理斑马鱼，从3 dpf处理到6 dpf，观察指标^[1]。

结果分析

造模成功指标：中性粒细胞数量增多、酸性粘液蛋白增多、炎性因子表达量上升。

1.嗜中性粒细胞数量：中性粒细胞数量增多，并且向肠和上皮中移动

2.酸性粘液蛋白：石蜡病理切片结合AB-PAS染色法验证，DSS浸泡后斑马鱼肠道中酸性粘液蛋白增多

3.炎性因子表达量：DSS处理后斑马鱼肠道中炎性因子表达量上升^[1,2]

客户案例

图1.Yeasen DSS（Cat#60316ES）斑马鱼造模实验结果（Jing Ma,et al. Aquaculture and Fisheries (2021) 548–557）

DSS处理组相较于对照组，酸性粘蛋白染色和粘液分泌增加，斑马鱼外周血中的嗜中性粒细胞数量增多并且向肠和上皮中移动，炎性因子表达量显著上调，肠道出现炎症反应。

翌圣DSS产品优势

翌圣提供高品质DSS（Cat.NO：60316ES，MW:36000~50000），具有以下优势：

1.高纯度（＞98%），硫含量17-19%，游离硫＜0.2%，大量数据文献支持，广泛应用于UC模型的构建；

2.多种属动物中均可造模：小鼠、大鼠、斑马鱼、猪、果蝇；

3.症状表现与人UC极度相似：可用于研究急、慢性结肠炎的发生发展机制，也可用于药物的药效研究；

4.简单易行，成模率高，重复性强；

5.联合氧化偶氮甲烷(azoxymethane, AOM)用药，可用于诱发结肠炎相关性癌症 (colitis associated cancer, CAC) 动物模型，成功模拟IBD诱发CAC的过程。

建模效率与进口产品一致，产品价格低至进口价格的1/3，大量现货库存。

产品订购

产品名称	货号	规格
ColitCare^®Dextran Sulfate Sodium Salt（DSS）结肠炎建模用葡聚糖硫酸钠盐 MW : 36000～50000	60316ES25	25 g

ColitCare^®Dextran Sulfate Sodium Salt（DSS）结肠炎建模用葡聚糖硫酸钠盐 MW : 36000～50000	60316ES60	100 g

ColitCare^®Dextran Sulfate Sodium Salt（DSS）结肠炎建模用葡聚糖硫酸钠盐 MW : 36000～50000	60316ES76	500 g

ColitCare^®Dextran Sulfate Sodium Salt（DSS）结肠炎建模用葡聚糖硫酸钠盐 MW : 36000～50000	60316ES80	1 kg

点击产品名称查看详情

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2022

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[4] JialiDong,YuanLi,HuiwenXiao,et al.Oral microbiota affects the efficacy and prognosis of radiotherapy for colorectal cancer in mouse models[J].Cell reports.2021, 109886.IF=9.423

[5] Yaohua Fan,Yanqun Fan,Kunfeng Liu,et al.Edible Bird’s Nest Ameliorates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice by Restoring the Th17/Treg Cell Balance[J].Frontiers in Pharmacology.2021.632602.IF=7.561

2020

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[2] Gao X, Fan W, Tan L, et al. Soy isoflavones ameliorate experimental colitis by targeting ERα/NLRP3 inflammasome pathways[J]. The Journal of Nutritional Biochemistry, 2020, 83.IF=6.048

[3] Li, Y., Dong, J., Xiao, H., Zhang, S., Wang, B., Cui, M., & Fan, S. Gut commensal derived-valeric acid protects against radiation injuries. Gut Microbes,.2020 .1–18.IF=10.245

BEFORE 2020

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