Article Detail
Case Studies同源重组克隆试剂盒案例│1800 bp单片段插入
📂 克隆系列 Type ID 5002
插入片段信息片段长度1800 bp片段浓度50 ng/μL片段纯化方式琼脂糖凝胶电泳回收载体信息载体长度7200 bp载体浓度20 ng/μL载体纯化方式琼脂糖凝胶电泳回收反应体系及程序组分体积(μL)2×HieffClone®Universal IIEnzymePremix5 μL片段1 μL载体4 μL无菌超纯水0 μL50℃反应5分钟。克隆生长情况阳性
Source: Yeasen Biotech | Original: https://www.yeasen.com/solutiondetail/2034510945350991872 ↗
插入片段信息
片段长度 | 1800 bp |
片段浓度 | 50 ng/μL |
片段纯化方式 | 琼脂糖凝胶电泳回收 |
载体信息
载体长度 | 7200 bp |
载体浓度 | 20 ng/μL |
载体纯化方式 | 琼脂糖凝胶电泳回收 |
反应体系及程序
组分 | 体积(μL) |
2×HieffClone®Universal IIEnzymePremix | 5 μL |
片段 | 1 μL |
载体 | 4 μL |
无菌超纯水 | 0 μL |
50℃反应5分钟。
克隆生长情况

阳性克隆鉴定结果

客户评价
文末福利
特别推荐
Hieff Clone® Universal II One Step Cloning Kit

Ø定向:可满足1-7个DNA片段的定向无缝拼接;
Ø简便:片段无需酶切,多片段连接一管内即可完成;
Ø快速:最快5分钟即可完成连接反应;
Ø高效:克隆生长数多,克隆阳性率可达95%以上;
Ø广泛:适用于任何载体,兼顾粘性和平末端;可用于多片段、点突变等的克隆;
Ø超长:载体片段连接长度可达25 kb以上。
点击试用装申请链接,搜索10923ES,即可申请免费获得产品试用装。
Source
Source:
Yeasen Biotech
Source ID: 2034510945350991872
Content is compiled from Yeasen Biotech public materials; all rights belong to the original author.
Products CoveredFrom source article
IMG
Hieff Canace®Veritas Long PCR Master Mix...
Not listed yet IMG 2× Hieff Canace® AdvanceFast PCR Master ...
Not listed yet IMG 2× Hieff Canace® Plus PCR Master Mix (Wi...
Not listed yet IMG 2× Hieff® Ultra-Rapid II HotStart PCR Ma...
Not listed yet IMG Hieff Clone® Universal II One Step Cloni...
Not listed yet IMG Hieff Clone® Universal One Step Cloning ...
Not listed yet IMG Hieff Clone® Plus One Step Cloning Kit 一...
Not listed yet IMG Hieff Clone® Universal Zero TOPO TA/Blun...
Not listed yet IMG Hieff Clone® Plus Multi One Step Cloning...
Not listed yet IMG 2× Hieff Canace® AdvanceFast PCR Master ...
Not listed yet IMG Hieff Canace®Veritas Long PCR Master Mix...
Not listed yet IMG 2× Hieff Canace® Gold PCR Master Mix 高保真...
Not listed yet IMG 2× Hieff Canace® PCR Master Mix 高保真酶预...
Not listed yet IMG Hieff Canace® Gold High-Fidelity DNA Pol...
Not listed yet IMG Hieff Canace® Plus High-Fidelity DNA Pol...
Not listed yet
Not listed yet IMG 2× Hieff Canace® AdvanceFast PCR Master ...
Not listed yet IMG 2× Hieff Canace® Plus PCR Master Mix (Wi...
Not listed yet IMG 2× Hieff® Ultra-Rapid II HotStart PCR Ma...
Not listed yet IMG Hieff Clone® Universal II One Step Cloni...
Not listed yet IMG Hieff Clone® Universal One Step Cloning ...
Not listed yet IMG Hieff Clone® Plus One Step Cloning Kit 一...
Not listed yet IMG Hieff Clone® Universal Zero TOPO TA/Blun...
Not listed yet IMG Hieff Clone® Plus Multi One Step Cloning...
Not listed yet IMG 2× Hieff Canace® AdvanceFast PCR Master ...
Not listed yet IMG Hieff Canace®Veritas Long PCR Master Mix...
Not listed yet IMG 2× Hieff Canace® Gold PCR Master Mix 高保真...
Not listed yet IMG 2× Hieff Canace® PCR Master Mix 高保真酶预...
Not listed yet IMG Hieff Canace® Gold High-Fidelity DNA Pol...
Not listed yet IMG Hieff Canace® Plus High-Fidelity DNA Pol...
Not listed yet
Related
- 📄【News】翌圣琼脂糖——直击电泳痛点,跑出一手好胶
- 📄【Literature】MolecularCell|陈玲玲:发现NF90/NF110对环形RNA表达调控和功能作用的新机制
- 📄【Literature】Science|王二涛课题组:植物-微生物互作的全新机制
- 📄【Literature】Cell|陈玲玲课题组:首次发现调控RNA聚合酶转录的lncRNA(附专家点评)
- 📄【Literature】PLoS Biology| 植生所晁代印研究组:揭示CTL1影响植物生长发育及离子平衡新机制
- 📄【Literature】PLoS Genetics|晁代印研究组:AtHKT1控制沿海拟南芥生态型适应高盐环境的新机制