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4ans

272views

For plasma TNF-α detection, should EDTA or heparin anticoagulant be used? Which kit should be selected for low-concentration samples?

Supplier & Sourcing TTomAssay 2025-11-25 #TNF-α ELISA #Plasma samples #Anticoagulant

Plant PDH E1αElisa K...

HMG-COA Elisa Kit

4ans

270views

What is the lower limit of linearity for the BCA kit for low-concentration proteins in fish serum?

Lab Techniques CCellLine3177 2026-08-09 #BCA Assay Kit #Fish serum #Muscle tissue

4ans

187views

Will adding penicillin-streptomycin to primary rat fibroblasts affect their growth?

Storage & Shipping RReagenting8982 2026-09-07 #Penicillin-Streptomycin #Primary cell culture #Cytotoxicity

4ans

271views

For animal experiments, is a DMSO concentration of 10% or 5% more appropriate for rat liver tissue cryopreservation solution?

Reagent Selection BBio 2026-04-24 #Dimethyl sulfoxide #Tissue cryopreservation #Rat

4ans

252views

What is the appropriate safe concentration of DMSO as a solvent for intraperitoneal injection in animal experiments using mice?

Lab Techniques 郑郑杰7662 2026-07-31 #Dimethyl sulfoxide #Animal experiments #Intraperitoneal injection

5ans

202views

When using BCA to quantify serum samples, low concentrations are always inaccurate. Are there any optimized conditions?

Reagent Selection JJack5561 2026-09-06 #Serum samples #Low-concentration protein #BCA quantification

4ans

217views

Can IPTG induce inclusion body proteins and increase concentration promote expression?

Supplier & Sourcing 邓邓检测 2026-04-29 #IPTG #Inclusion bodies #Concentration optimization

4ans

235views

Is the linear range of BCA assay kit sufficient for precise protein quantification before antibody conjugation?

Lab Techniques 胡胡晨 2026-03-10 #BCA method #Antibody quantification #High-concentration samples

4ans

272views

How to optimize the concentration and time of EDTA free trypsin for primary neuron digestion?

Compliance PPCRPro1352 2026-08-03 #EDTA-free Trypsin #Primary cells #Cell dissociation

3ans

226views

Can different brands of fetal bovine serum maintain hybridoma cells at a concentration of 2%?

Compliance AAssay4380 2026-07-26 #Hybridoma cells #Fetal Bovine Serum #Added concentration

3ans

278views

Is 5% fetal bovine serum sufficient for primary neuron culture?

Storage & Shipping 宋宋明明4572 2026-08-03 #Primary neurons #Fetal Bovine Serum #Low-serum culture

3ans

259views

Is 10% or 15% fetal bovine serum more suitable for raising MSCs?

Reagent Selection JJackBuffer5621 2026-08-23 #Mesenchymal stem cells #Fetal Bovine Serum #Added concentration

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