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274views

His-tagged inclusion body protein purification: How to choose between Ni-NTA and TALON resins?

Lab Techniques 杜杜阳 2025-10-13 #Protein purification #His tag #Nickel column

Recombinant Biotinyl...

0ans

240views

How should I choose a pre-packed column for His-tagged protein purification? Please provide a sales quotation and selection recommendations.

Storage & Shipping 何何科研 2025-12-17 #Protein purification #His tag #Pre-packed column

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3ans

179views

His-tagged protein purification, seeking recommendations for nickel column prepacked columns with high loading capacity and anti-clogging properties.

Lab Techniques KKiting 2025-11-12 #Protein purification #His tag #Nickel column

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5ans

254views

After purification, the His-tagged protein is quantified using BCA. At what imidazole concentration will it interfere?

Medical Devices 许许艳燕6255 2026-09-09 #His-tagged protein #Imidazole interference #BCA quantification

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5ans

190views

After purifying a His-tagged recombinant protein, if you want to cleave off the tag, is TEV or thrombin more cost-effective?

Compliance RReagentHub3082 2026-09-04 #Recombinant protein purification #His tag #Protease cleavage

Recombinant Biotinyl...

4ans

196views

After purification of His-tagged recombinant protein using a nickel column, the bands are impure. How can I optimize the conditions?

Compliance AAndy2058 2026-08-07 #Recombinant protein #His tag #Nickel column purification

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3ans

250views

The endotoxin level of His-tagged recombinant protein is relatively high. How can it be removed in the laboratory?

Supplier & Sourcing 唐唐采购8407 2026-08-27 #Recombinant protein #His tag #Endotoxin

Plant NF-κBElisa Kit

Recombinant Biotinyl...

4ans

305views

How effective is the purification of His tagged proteins using pre installed chromatography columns from domestic biosensing brands?

Lab Techniques BBuffer968 2026-06-26 #Protein purification #His tag #Pre-packed chromatography column

Recombinant Biotinyl...

4ans

236views

What is the loading capacity for purifying His tagged protein using domestically produced Ni NTA fillers?

Medical Devices RResearchPlus 2026-08-01 #Protein purification #His tag #Ni-NTA

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