How to choose between domestic and imported mouse serum IL-6 ELISA kits?

In practice, 40 samples were tested for two indicators simultaneously. It is recommended to use the same brand and batch of reagent kits for IL-6 and TNF - α, with consistent operating conditions and washing steps to reduce data fluctuations. When purchasing, ask about the spot batch, validity period and whether Flat noodles can be separated, so that if the test is not completed at one time, the plate can be sealed for next use. The first thing to do upon arrival is not to directly load all samples, but to confirm the color development time and sample dilution factor using standard samples and 3-5 pre test samples, as there may be individual differences in mouse serum matrix. If the nominal sensitivity of domestically produced boxes is sufficient, small samples can be run first, and if the batch is found to be unstable, imported key samples can be considered for supplementation.

The difference between domestic and imported can not only look at the sensitivity of the instructions. In recent years, the difference between domestic inflammation indicators on routine serum samples has not been large, but the stability between batches still needs to be measured. It is recommended that you ask the supplier to provide two bags or small samples of the same batch number, and run a standard curve with the same mixed serum to see the CV and recovery rate; if the sample is precious, you can dilute it with the standard first. The control between import boxes is usually more mature, but there are few common specifications within 1500, so you may need to wait for activities or find a regional agent. Prioritize brands that can arrive in the cold chain within a week, have literature citations, or after-sales technical support, and do not choose a completely unverified brand for saving dozens of dollars.

When using 96T for 40 samples, it is important to calculate the number of wells: if you are doing a single well, removing the standard curve and blank space is generally sufficient; However, it is recommended that serum samples be re pooled at least. IL-6 and TNF - α are also susceptible to hemolysis and freeze-thaw effects, and 40 samples may not be enough after re pooling. It is suggested to split the samples into two boxes or confirm with the agent whether they can be disassembled. Priority should be given to domestic stock within a budget of 1500, but it is necessary to inquire whether standard products are ready to use and whether they include pre packaged boards. After arrival, take 2-3 samples for low and high value mark up recycling, and then decide whether to replenish.