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All Answers2
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TomElisa
25 days ago
小样本量项目我会优先选验证充分的ELISA，并把变量压到洗板和标准曲线上：固定孵育温度时间、优化洗板循环、做板内板间重复性。化学发光确实对手动操作友好，但仪器不稳定时结果波动会更隐蔽，不适合刚起步项目。选试剂盒重点看定量线性、检测下限、基质效应、参考品可溯源，以及能否用自家样本完成方法学验证。
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小小老司机
23 days ago
如果结果要用于疫苗评价或横向比较，化学发光的宽线性、自动化洗板和高通量优势会更明显，前提是实验室有稳定设备和维护能力。ELISA成本更可控，适合样本量小、偶发项目。实际选型建议算全成本：试剂、校准品、仪器维护、人员时间；再做一批样本双方法比对，看临界值附近一致性和CV，而不是只比较单价。
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