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All Answers2
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RexAssay
23 days ago
自采保存液和PCR管是否兼容,关键不在管本身,而在胍盐、灭活剂、醇类、EDTA或拭子基质是否被提取步骤去除干净。若磁珠/柱提充分清洗、乙醇挥发或膜平衡到位,常规八联管或96孔板通常可兼容;若残留盐分或乙醇进入反应,可能导致Ct升高、内标失败、弱阳性或非预期结果。建议用目标拭子加保存液做加标回收、稀释度、阴/阳对照和IPC Ct验证,同时确认PCR管无DNase/RNase、低吸附、热盖和荧光透过匹配仪器。社区项目尽量选采样包、保存液、提取方案和检测试剂盒同品牌闭环。
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Science5340
25 days ago
首批1000人份可拆成3批试做,先索取试用装或小包装96T/192T做性能验证。常规注册产品有库存时货期常见3-7个工作日,组合采样包、特殊保存液、定制包装或注册规格调整可能需要2-3周;社区送检还要预留运输温度、时效和到货验收。选型维度看:是否有NMPA注册、是否做过自采样本验证、报告分型需求、保存液是否含胍盐灭活、提取兼容、是否提供阴阳性参考品和内标、能否稳定供货。若担心跨品牌保存液,最好同品牌闭环,或自行做保存液A提取后进入B检测的交叉验证。
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