More serum samples, more reliable H. pylori test kit with ELISA or C13 exhalation

If the goal of the project is to determine the current infection, IgG ELISA alone is not enough, the antibody may continue to be positive after previous infection, and it is easy to treat historical exposure as a current infection. It is safer to stratify: ELISA is initially screened, and then the key or gray area samples are combined with the C13 breath test; the pairing consistency, Kappa and yin and yang prediction values are done at the time of statistics, and the difference in the intended use of the two methods is stated in the protocol.

Running 96 wells per day to control the cost, the key is not to press only the unit price of the reagent, but to reduce the failure hole and rework. It is recommended to complete 800 copies of the same batch, and place them separately to avoid edge effects; each plate is equipped with yin-yang control and quality control holes, and lipid blood samples are first evaluated for interference. Suppliers can provide trial boards or evaluation packages, measure the repetition in the board and the CV between the boards, and then decide whether to purchase the whole board.