Antigen detection kits are used for clinical samples, how to choose the sensitivity difference between different manufacturers?

Clinical sample antigen testing is critical for pretreatment compatibility. When selecting the type, confirm whether the swab material, preservation solution, and lysis solution affect the chromatography, especially the interference of protein- or polymer-containing samples. Check whether the card case, membrane material, and reading mode are compatible with existing pipettes, centrifuges, and readers. Stability and consumable compatibility are more important than the price of a single box: it is recommended to do a batch continuous arrival simulation, check the expiration date, transport temperature control, and interpret the window drift after opening the card. The daily amount can be added to the main supplier to reduce the risk of method switching due to batch number differences or material supply breaks.

First of all, don't just focus on the LOD numbers sold to you. The calibers of different manufacturers vary greatly. It is recommended to request a clear limit of detection method in the registration or filing information: virus strain, matrix, dilution gradient, whether to use TCID50 or RNA copy, and request the basis for conversion. Weakly positive retained samples are blindly tested internally, covering samples close to LOD, comparing negative quality control, matrix effect and repeat test consistency. 200 cases can establish a monthly stability ledger every week, separating the false negative rate, retest rate and sample type. Priority is given to manufacturers with transparent methodology, complete inter-batch reports, and on-site verification.