Looking for recommendations for a porcine reproductive and respiratory syndrome antibody detection kit, testing 300 serum samples per week?

From a procurement and cost perspective, 300 units per week, occasionally 500 units, should preferably be calculated by the full plate count. The per-well cost of imported kits is usually higher than that of domestic ones, but there are fewer complaints about batch-to-batch variation; domestic products have improved in stability in recent years and have advantages in price and delivery lead time. It is recommended that distributors quote both "spot" and "forward" prices at the same time, and clearly ask about the temperature records upon opening after cold-chain delivery and the remaining months of shelf life. For small-package trials, do not just ask whether they are available; confirm whether the trial pack includes positive and negative controls and whether it can be used for batch-to-batch validation. Signing an annual framework agreement with delivery in batches can reduce inventory shelf-life risk. For classical swine fever and pseudorabies, check whether the same supplier can cover both, to avoid the acceptance hassle caused by sourcing from three suppliers. Although multiplex kits are more convenient, in pig farm monitoring, when false-positive or suspicious samples need retesting, single-target kits are clearly more flexible.

For serological monitoring on pig farms, don't rush to switch brands. It is advisable to first request small samples from 3 different batches from the current manufacturer or new candidate manufacturers, and use 20-30 known serum samples (including negative, positive, and weak positive) for parallel comparison. Focus on intra-assay CV, inter-assay CV, and the distribution of S/P values, rather than only looking at positive/negative agreement rates. Imported PRRS antibody kits such as IDEXX and Biochek have relatively stable quality control, but the unit price is higher and lead times are longer, with futures often requiring 4-8 weeks; leading domestic manufacturers such as Jinnuo and Keqian generally have lead times of 1-2 weeks and can support small-package trials. For 300-500 samples per week, it is recommended to prioritize 96-well detachable plates to facilitate batch operation. For classical swine fever and pseudorabies, it is recommended to purchase separate single tests. Test classical swine fever with a blocking ELISA, and test pseudorabies gB/gE separately. This makes the monitoring purpose clearer, whereas combined testing is instead not conducive to troubleshooting.

When our laboratory switches reagent kits, we run approximately 30 retained samples with both the old and new kits, including clinically suspected, weakly positive, and samples from different cycles after vaccine immunization, to calculate the consistency of qualitative and quantitative results before deciding whether to switch. For PRRS ELISA, the focus is on the positive control/weak positive quality control. If the weak positive wells drift significantly, marginal well abnormalities are very likely to occur in subsequent large-volume sample testing. Plate washing and incubation times must strictly follow the instructions, especially when the room temperature is below 20°C. Regarding brands, I have experience with IDEXX, Biochek, and domestic brands such as Jinnuo and Laipusheng, all of which have regional agents that can provide trial samples. It is recommended to ask manufacturers to provide COA for three production batches and inter-batch variation data. For classical swine fever and pseudorabies, single-plex testing is recommended, performing blocking and gE antibody assays separately. In particular, pseudorabies gE is used for wild-type virus screening, and single-plex testing is more aligned with eradication and surveillance needs.