Question Detail
For digesting mouse adipose-derived stem cells, is collagenase or a mixed enzyme better?
Reagent Selection
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All Answers4
B
Bio8967
2026-09-18
Liberase或同类混合酶的优势是批间稳定、对细胞表面抗原保留更好，适合下游流式、单细胞测序或需要重复性的实验；缺点是价格高、货期要询代理，一般比单胶原酶贵。经费有限就把它留给正式实验，国产或普通胶原酶做预实验筛选条件。
试
试剂老张
2026-09-16
胶原酶+分散酶通常比单用胶原酶更适合脂肪组织，分散酶帮助解离基底膜和ECM，胶状物会少一些。我一般用胶原酶I或II为基础，加分散酶和DNase I，37℃摇40 min左右，之后用100 µm和40 µm滤网过滤，离心去油。活率和得率要放在同一脂肪量下比，不然很难评价。
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Flask2292
2026-09-12
建议设置三组平行：单胶原酶+DNase I、胶原酶+分散酶+DNase I、Liberase+DNase I，固定脂肪量、小鼠年龄/性别、消化体积、摇速和终止条件。比较活率、贴壁率、成脂/成骨分化或表面标记，别只看一个时间点。价格和货期方面，进口混合酶小包装通常1-4周，具体要问代理商，不要按网价备货。
抗
抗体研究员
2026-08-01
小鼠腹股沟脂肪提干细胞，单用胶原酶1小时得率低、胶状物多，常见原因是DNA释放和脂肪未充分剪碎，不一定是酶种类问题。建议脂肪剪成乳糜状，加DNase I，消化30-45 min，中间轻柔摇晃，不要等1小时到点才看。胶原酶浓度过高或时间过长都会压活率。
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