Question Detail
Does the PBS used for washing tissues in the animal room have to be purchased as sterile and calcium- and magnesium-free?
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Tags：#Phosphate buffer solution #Animal experiments #Tissue sampling #Sterile PBS #Procurement Inquiry
All Answers4
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WangResearch
2026-09-09
验收和保存要留意：即用型PBS到货后先看瓶身完整性、有效期、有无沉淀；开瓶后记录日期，无菌PBS开瓶后不建议长期反复用。如果自己配干粉，建议用注射用水或超纯水，灭菌后复测pH，正常在7.2-7.6左右可接受。供应商方面，可比较是否有COA、是否提供内毒素检测报告、批间一致性，别只看单价。
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LisaMedia
2026-09-17
从成本看，一次500 mL，如果每周几次实验，买即用型500 mL/瓶最省事，开封后4℃保存注意避免污染；如果月用量大，买干粉自己配可以降成本，但要配超纯水、调pH、高压或过滤，普通动物房容易操作出错。货期上，国产现货常见3-7天，进口或冷门配方可能要1-2周，建议常备1-2瓶库存，不要等用完再补。
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SamMedia
2026-07-02
如果是取脾脏和淋巴结，后续可能做淋巴细胞分离、流式或原代培养，建议优先选无钙镁PBS。钙镁离子会让细胞更容易黏附聚集，冲洗时红细胞也不易散开；如果只是提RNA或做固定，普通PBS影响不大。无菌不是绝对必须，但新鲜组织本身不是无菌，冲洗液若带菌可能增加污染变量，所以后续只要涉及培养，就按无菌买更省心。
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AmyBio
2026-09-13
你朋友说的普通PBS就行，在只冲洗血污、不做培养的场景确实可以；但专业老师建议无钙镁是考虑到取样后细胞活率和后续酶消化。建议按实验目的分两档：组织学或分子实验可用普通PBS；免疫细胞分离、原代培养、单细胞测序用无钙镁无菌。购买时核对标签是否写without Ca/Mg，而不是只看PBS。
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