Will the fluctuation of endotoxin levels in fetal bovine serum from different batches of the same brand affect 293T transfection?

<p>293T cells are indeed less sensitive to LPS than primary macrophages, but when endotoxin levels rise, cells may show deteriorated condition, poor adherence, and increased mortality after transfection. In your report this time, you only wrote</p>

<p>For a weekly usage of 200 mL, the biggest concern is using a new lot directly without verification after a lot change. Going forward, a three-step approach can be followed: first, sample upon arrival and quantify endotoxin using LAL reagent; at the same time, use 293T to perform a 48-hour growth curve; then perform a transfection efficiency control. Only after all three items pass should use be scaled up. Alternatively, based on usage, 6-10 bottles from the same lot can be locked in at one time to reduce the frequency of lot changes. For reports, only write</p>

<p>It is very common for endotoxin levels in different batches of serum to fluctuate, especially when the supplier&#039;s report only states</p>