Buy FFPE chip to measure immunohistochemical antibody concentration, do you have clinical staging data?

For PD-L1 condition groping, it is recommended to select a chip of more than 2 mm with TNM staging and pathological grading annotation, preferably including pairs of primary foci and lymph node metastases. This kind of spot is often found in lung cancer, gastric cancer, esophageal cancer, etc., but the number of lymph node metastasis points is limited. Ask about the time of lymph node acquisition and the fixation conditions. 50 repeated slices on a 2mm chip are generally acceptable, but it is recommended that the supplier promises to cut the number of sheets and do the first and last HE quality control to avoid cutting to the back tissue loss. The price and delivery time depends on whether there is stock, the stock is usually shipped within 1-2 weeks, and the customization may be 3-5 weeks.

50 sheets are not a small number, it is recommended to cut them in two batches: first cut 20 sheets for concentration gradient pre-test, and then cut 30 sheets to avoid antigen attenuation after one cut. PD-L1 staining is sensitive to the freshness of the slices. Stain as soon as possible after arrival, and store the temporarily unused slices at -20°C. If the supplier provides a PD-L1 reference staining chart, it depends on whether the clone number, platform and scoring criteria are consistent with your laboratory. The price of chips with clinical phasing will be higher, the more expensive the clinical data is, but the cost of 50 chips is controllable.

I chose this type of chip to focus on whether the clinical information is standardized, whether the TNM staging is version 8 or version 7, whether the grading is clear, and whether the lymph node metastases are independent points or paired with the main lesions. You can have sales send out a desensitization datasheet template to confirm if the field contains gender, age, staging, grading, sampling site. If the lymphoid foci are mixed in the primary foci chip, the coordinates should be marked separately. Regular companies can cut 50 sheets for repeated slices, but it is recommended to ask about the depth of trimming and anti-stripping treatment. The delivery period shall not be subject to verbal commitments, and shall be subject to confirmation by mail.

In the antibody concentration exploration stage, it is recommended to do a small gradient pre-test with 4-6 points on the chip first, not to use a full sheet. The effect of tissue heterogeneity can be reduced by comparing different concentrations with continuous slice ratios. If the chip carries lymph node metastases, these points can be arranged separately as a positive/negative control to help determine staining specificity. In terms of price, chips containing TNM and lymph node metastases are usually priced on a per-sheet basis, and an increase in the number of slices will affect the total price, but repeated slices are generally counted separately by the number of sheets, for specific inquiries. In the delivery period, the spot is generally faster, and the customization or semi-customization should be reserved for a longer time.