Doing IHC pre-experiments Looking for an FFPE chip with normal and tumor pairs, are there any in stock?

Do not only focus on the price and cancer type in the pre-test, first ask the chip array diagram, the slice thickness, whether the anti-detachment treatment is performed, and whether the HE reference diagram is taken. The small dot diameter of 1.5mm is easy to drift or the edges are uneven in IHC. It is recommended to select a positive charge strip and dry the sheet first after receiving it. If the supplier has undyed white sheets, you can buy a few sheets of test dye first, do not buy the whole sheet. Small chips at 20-30 points are generally priced per sheet. The specific price varies greatly depending on the type of cancer and the pairing design. When requesting quotations, confirm the "tax-included transportation, the number of slices, and the storage conditions" together to avoid subsequent increases.

If you want it this week, it is recommended to ask 2-3 Shanghai or East China spot suppliers at the same time, request the other party to provide the spot number, dot diagram and HE thumbnail, and pay the same day after confirmation. Pairing chips at 20-30 points occasionally occur, but common cancers such as breast cancer, colorectal cancer, liver cancer are more common, and lung cancer, stomach cancer, etc. depend on inventory. After receiving it, first check whether the slice is complete, especially the 1.5mm point is easy to be missing, and the problematic point is photographed and left in time for feedback. The price of this block, the small specification spot is usually lower than the customization, but the pairing and clinical information will increase the cost, depending on the quotation.

It is recommended to make the sales distribution inventory table first, focusing on whether there is a small specification chip with "cancer + cancer pairing". The specification of 1.5mm diameter and 20-30 points is not much in stock. Common hepatocellular carcinoma, lung adenocarcinoma, colorectal cancer, breast cancer, etc., but the inventory changes greatly. The price is mainly influenced by points, pairing design, tissue source and number of slices, and pairing chips are usually more expensive than single cancers. If it is the same city in Shanghai, after confirming the spot delivery or SF, you can arrive within 1-2 working days. This week's delivery depends on whether the other party can leave the warehouse on the same day. It is recommended to ask the date of delivery directly, rather than just "available in stock".

From the perspective of supplier comparison, I generally look at three dimensions: first, whether to provide the tissue type and pairing relationship at each point, and how far away the cancer is from the tumor; second, whether there is an HE or immunohistochemical reference map, and whether the tissue shape can be seen in advance; third, the delivery time of the spot, Shanghai local companies can ask whether they support self-pickup or same-city flash delivery. If this specification is to touch the antibody conditions, it is recommended to choose a multi-cancer pairing chip, with a higher flux. There may be few pairs of 20-30 points for a single cancer species, and the cost performance is average. Don't just look at the quotation on the price, confirm the freight and special tickets.