Lab Techniques - BioReagent Hub

5ans

201views

Must the medium be switched to exosome-depleted fetal bovine serum for extracellular vesicle isolation?

Lab Techniques WWendy432 2026-09-10 #Fetal Bovine Serum #Exosomes #Ultracentrifugation

5ans

258views

After thawing fetal bovine serum, if white flocculent precipitate appears, can it still be used?

Lab Techniques 孙孙鹏 2026-09-09 #Fetal Bovine Serum #Primary culture #Serum precipitate

5ans

207views

What should be done if hybridoma antibody yield fluctuates greatly after changing the fetal bovine serum lot?

Lab Techniques 赵赵静燕 2026-09-24 #Fetal Bovine Serum #Hybridoma #Antibody production

5ans

193views

Immunofluorescence double staining requires primary antibodies from different species. How can cross-reactivity be assessed?

Lab Techniques 试试剂同学9920 2026-09-03 #Immunofluorescence #Primary antibody #Cross-reactivity

Recombinant Alexa Fl...

5ans

226views

For immunohistochemistry on paraffin sections, is it more cost-effective to use ready-to-use or concentrated primary antibodies?

Lab Techniques 飞飞飞大姐 2026-09-05 #Immunohistochemistry #Primary antibody #Paraffin section

5ans

221views

For WB detection of a phosphorylated protein in mouse liver tissue, how should the primary antibody be selected?

Lab Techniques KKevin5578 2026-09-17 #Western blot #Primary antibody #Mouse tissue

5ans

233views

When using high-fidelity enzymes for site-directed mutagenesis, what should I do if the mutation efficiency remains low?

Lab Techniques NNina6324 2026-08-26 #High-fidelity enzyme #Site-directed mutagenesis #Plasmid PCR

5ans

234views

Procurement inquiry: How are the lead times for imported reverse transcriptase kits recently? Are there any reliable domestic alternatives?

Lab Techniques 杨杨小妹6241 2026-09-22 #Reverse transcriptase #Lead time #Domestic substitution

4ans

185views

For culturing HepG2 and RAW264.7, should DMEM high glucose or 1640 be used? Is glutamine supplementation needed?

Lab Techniques AAndyProtein 2026-04-25 #Cell culture medium #DMEM #RPMI1640

Recombinant SARS-COV...

Recombinant SARS-Cov...

4ans

231views

How to choose Oligo dT resin for in vitro transcribed mRNA vaccine purification?

Lab Techniques 张张磊6040 2026-08-18 #mRNA vaccine #In vitro transcription #Purification resin

4ans

165views

For PD-L1 antibody immunohistochemistry in mouse tumors, which clone and retrieval conditions are recommended?

Lab Techniques AAssay3599 2026-09-05 #Immune checkpoint #PD-L1 antibody #Immunohistochemistry

4ans

247views

Looking for recommendations for IL-6 recombinant protein for use in ELISA standard curves. Is the activity consistent across batches?

Lab Techniques LLisaReagent5658 2026-09-02 #Recombinant protein #ELISA #IL-6

ELISA试剂盒

Plant PDH E1αElisa K...

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