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5ans

182views

After purifying a His-tagged recombinant protein, if you want to cleave off the tag, is TEV or thrombin more cost-effective?

Compliance RReagentHub3082 2026-09-04 #Recombinant protein purification #His tag #Protease cleavage

Recombinant Biotinyl...

5ans

208views

Can flow cytometry antibodies for surface staining be used for intracellular staining after fixation and permeabilization?

Compliance DDavidScience1321 2026-08-30 #Flow cytometry antibodies #Intracellular staining #Th17

Recombinant Biotinyl...

5ans

264views

Has anyone compared prices for switching the IHC secondary antibody from a universal type to an anti-rabbit/mouse dual-label one?

Supplier & Sourcing RReagentPlus3077 2026-09-01 #Immunohistochemistry #IHC secondary antibody #Double-labeled

5ans

212views

How should the remaining wells of an ELISA kit microplate be stored after stripping the strips? Is the inter-batch variation large?

Compliance DDavid 2026-08-27 #ELISA kit #Microplate #Strip

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

313views

The sample volume of cell culture supernatant is very limited. What is the minimum volume in microliters that can be used with the ELISA kit?

Supplier & Sourcing 余余洋辉 2026-09-07 #ELISA kit #Cell supernatant #Small sample

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

210views

For batch testing of rat TNF-α, how do the lead time and price of a 96T ELISA kit compare?

Storage & Shipping 杨杨娜2860 2026-09-12 #ELISA kit #TNF-α #Rat

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

322views

For human IL-6 ELISA kits used to measure serum samples, are there large differences in recovery rates among different brands?

Compliance VVivi3152 2026-08-31 #ELISA kit #IL-6 #Serum samples

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

236views

When using high-fidelity enzymes for site-directed mutagenesis, what should I do if the mutation efficiency remains low?

Lab Techniques NNina6324 2026-08-26 #High-fidelity enzyme #Site-directed mutagenesis #Plasmid PCR

5ans

206views

When using the ABI StepOnePlus for mouse gene expression, how should I choose between ROX and dye-based methods?

Reagent Selection 董董飞飞 2026-09-18 #qPCR kit #ROX #ABI StepOnePlus

Recombinant SARS-CoV...

4ans

191views

For culturing HepG2 and RAW264.7, should DMEM high glucose or 1640 be used? Is glutamine supplementation needed?

Lab Techniques AAndyProtein 2026-04-25 #Cell culture medium #DMEM #RPMI1640

Recombinant SARS-COV...

Recombinant SARS-Cov...

4ans

221views

When using a certain imported serum-free medium for Expi293 suspension culture, cell clumping always occurs. Seeking an alternative solution.

Storage & Shipping OOscarLab 2026-09-07 #Cell culture medium #Serum-free medium #Suspension culture

4ans

232views

How to choose Oligo dT resin for in vitro transcribed mRNA vaccine purification?

Lab Techniques 张张磊6040 2026-08-18 #mRNA vaccine #In vitro transcription #Purification resin

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