Q&A Community - BioReagent Hub

5ans

219views

Which high-fidelity enzyme is stable for amplifying long fragments? Can it amplify fragments over 8 kb?

Reagent Selection TTube30 2026-09-13 #High-fidelity enzyme #Long-fragment amplification #Genomic template

5ans

239views

When using high-fidelity enzymes for site-directed mutagenesis, what should I do if the mutation efficiency remains low?

Lab Techniques NNina6324 2026-08-26 #High-fidelity enzyme #Site-directed mutagenesis #Plasmid PCR

5ans

253views

High-fidelity enzyme amplification of high-GC templates always results in smearing. How can the system be optimized?

Supplier & Sourcing EEmmaTube4361 2026-09-24 #High-fidelity enzyme #High GC template #System optimization

5ans

196views

Which company offers the best cost-performance ratio for high-fidelity enzymes when amplifying 2 kb cDNA fragments?

Reagent Selection 唐唐博 2026-08-30 #High-fidelity enzyme #Molecular cloning #cDNA amplification

5ans

227views

Will adding too much reverse transcriptase inhibit downstream qPCR? How many units are generally appropriate to add to a 20 µL system?

Storage & Shipping NNinaAssay36 2026-09-13 #Reverse transcriptase #RT-qPCR #System optimization

5ans

185views

When performing RNA reverse transcription from plant leaves, inhibition often occurs. Which reverse transcriptase has strong resistance to polysaccharides and polyphenols?

Reagent Selection BBufferLab6335 2026-09-16 #Reverse transcriptase #Plant RNA #Polysaccharides and polyphenols

5ans

241views

Procurement inquiry: How are the lead times for imported reverse transcriptase kits recently? Are there any reliable domestic alternatives?

Lab Techniques 杨杨小妹6241 2026-09-22 #Reverse transcriptase #Lead time #Domestic substitution

5ans

231views

For reverse transcription of low-copy genes, is ordinary M-MLV or thermostable reverse transcriptase more suitable?

Storage & Shipping 唐唐敏 2026-08-29 #Reverse transcriptase #Low-copy gene #cDNA synthesis

5ans

246views

Fecal microbial DNA extraction, insufficient lysis leads to low yield. Any recommendations?

Compliance 周周采购 2026-09-20 #Fecal sample #Microbial DNA #Lysis

5ans

227views

For bulk packaging procurement of whole blood DNA extraction kits, which supplier has a faster delivery time?

Compliance MMedia 2026-08-26 #Whole blood DNA #Bulk packaging #Lead time

5ans

221views

For serum viral nucleic acid extraction, which magnetic bead-based method offers more stable recovery rates?

Reagent Selection TTom6502 2026-09-20 #Magnetic bead method #Viral nucleic acid #Serum and plasma

5ans

170views

Low purity in plant leaf RNA extraction and interference from polysaccharides and polyphenols: what should be done?

Compliance TTony4572 2026-09-19 #RNA extraction #Polysaccharides and polyphenols #Plant sample

Community Rules

1SearchAvoid duplicates

2Be SpecificContext + tries + errors

3Answer WellGive steps and evidence

4AcceptBest answer first

Incentives

✓Points for accepted answers

✓Top answerers listed

✓Vendor support on board

✓Auto-linked products

Community Q&AQ&A CENTER

Community Rules

Incentives