Q&A Community - BioReagent Hub

3ans

228views

After reconstitution, will the activity of the recombinant protein significantly decrease if stored at 4°C for one week?

Reagent Selection 罗罗娜 2026-08-29 #Recombinant protein #Cell culture #Storage conditions

3ans

249views

For using recombinant proteins as ELISA standards, is there a big difference between domestic and imported ones?

Supplier & Sourcing 马马磊5023 2026-09-19 #Recombinant protein #ELISA #Reference standard

Plant PDH E1αElisa K...

HMG-COA Elisa Kit

3ans

288views

When purchasing flow cytometry antibodies, is it necessary for the isotype control to be from the same brand and have the same fluorescence as the primary antibody?

Reagent Selection LLisa 2026-09-04 #Flow cytometry antibodies #Isotype control #Regulatory T cells

3ans

248views

After fixation and permeabilization, which flow cytometry antibodies can still be used to stain intracellular markers? Please recommend compatible fluorochromes.

Supplier & Sourcing AAmy9320 2026-09-22 #Intracellular flow cytometry #Fixation and permeabilization #IL-17A

3ans

192views

What are the appropriate cell and antibody amounts for flow cytometry antibody titration?

Storage & Shipping DDavid5521 2026-09-04 #Flow cytometry antibodies #Antibody titration #PBMC

Recombinant Biotinyl...

3ans

233views

For multicolor flow cytometry of mouse splenocytes, how should CD3/CD4/CD8 antibodies be paired with fluorophores to avoid interference?

Storage & Shipping RReagentLine8420 2026-09-07 #Flow cytometry antibodies #Multi-color color scheme #Mouse lymphoma

Recombinant Biotinyl...

3ans

190views

How is the probe uniformity of a targeted sequencing panel for genetic diseases evaluated? Is additional experimentation needed?

Medical Devices 李李采购5098 2026-08-27 #Genetic Disease Panel #Probe uniformity #Off-target

3ans

217views

How effective is cfDNA targeted sequencing panel for detecting low-frequency mutations?

Lab Techniques LLisaScience 2026-09-14 #cfDNA #Targeted sequencing panel #Low-frequency mutation

3ans

211views

How long does it generally take to design and synthesize probes when customizing a hematologic oncology panel yourself?

Supplier & Sourcing 彭彭辉 2026-09-07 #Custom Panel #Probe synthesis #Hematologic malignancies

3ans

320views

Which provider offers the best value for tumor-targeted sequencing panels? It needs to cover fusions and CNVs.

Compliance 大大师老司机3253 2026-08-30 #Targeted sequencing panel #Tumor gene testing #FFPE samples

3ans

203views

Is there a big difference in sensitivity between domestic and imported FISH hybridization kits, given that the price differs by a factor of two?

Lab Techniques DDavid 2026-09-12 #FISH #HER2 #Domestic vs. imported comparison

3ans

136views

Does the proteinase K digestion condition in the in situ hybridization kit need to be adjusted according to tissue type?

Storage & Shipping MMedia 2026-09-17 #In situ hybridization #Whole embryo #Proteinase K

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