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4ans

253views

Looking for recommendations for IL-6 recombinant protein for use in ELISA standard curves. Is the activity consistent across batches?

Lab Techniques LLisaReagent5658 2026-09-02 #Recombinant protein #ELISA #IL-6

ELISA试剂盒

Plant PDH E1αElisa K...

4ans

192views

After purification of His-tagged recombinant protein using a nickel column, the bands are impure. How can I optimize the conditions?

Compliance AAndy2058 2026-08-07 #Recombinant protein #His tag #Nickel column purification

Recombinant Biotinyl...

5ans

244views

For transfecting primary neurons with siRNA, are there any recommended transfection reagents with low cytotoxicity?

Compliance LLilyBuffer2727 2026-09-15 #Primary neurons #siRNA #Cytotoxicity

5ans

189views

The mouse serum sample volume is limited, and I want to measure multiple cytokines in one run. Should I choose CBA or Luminex?

Medical Devices 研研发达人2657 2026-09-08 #Multiplex assay #Cytokine #Flow cytometry

Plant PDH E1αElisa K...

HMG-COA Elisa Kit

5ans

231views

Our lab wants to purchase IL-6 and TNF-α ELISA kits. How should we choose between domestic and imported options?

Storage & Shipping 冯冯检疫8994 2026-09-07 #ELISA #Cytokine #Kit price comparison

ELISA试剂盒

Plant PDH E1αElisa K...

5ans

151views

Plasma exosome extraction: can it be done with a 200 µL sample? Looking for a low-sample-volume protocol.

Medical Devices 蒋蒋宇洋 2026-09-08 #Plasma #Exosome extraction #miRNA

5ans

239views

Are there exosome RNA liquid biopsy kits suitable for small-volume serum?

Medical Devices 蔡蔡斌 2026-09-11 #Exosomes #Liquid biopsy reagents #miRNA

3ans

249views

The endotoxin level of His-tagged recombinant protein is relatively high. How can it be removed in the laboratory?

Supplier & Sourcing 唐唐采购8407 2026-08-27 #Recombinant protein #His tag #Endotoxin

Plant NF-κBElisa Kit

Recombinant Biotinyl...

3ans

247views

For using recombinant proteins as ELISA standards, is there a big difference between domestic and imported ones?

Supplier & Sourcing 马马磊5023 2026-09-19 #Recombinant protein #ELISA #Reference standard

Plant PDH E1αElisa K...

HMG-COA Elisa Kit

3ans

246views

After fixation and permeabilization, which flow cytometry antibodies can still be used to stain intracellular markers? Please recommend compatible fluorochromes.

Supplier & Sourcing AAmy9320 2026-09-22 #Intracellular flow cytometry #Fixation and permeabilization #IL-17A

3ans

201views

Is there a big difference in sensitivity between domestic and imported FISH hybridization kits, given that the price differs by a factor of two?

Lab Techniques DDavid 2026-09-12 #FISH #HER2 #Domestic vs. imported comparison

3ans

244views

For paraffin section FISH, please recommend a digoxigenin-labeled hybridization kit with low background.

Supplier & Sourcing CCindyMedia9676 2026-08-29 #FISH #Paraffin section #Digoxigenin labeling

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