CFDA SE Cell Proliferation and Cell Tracking Kit 细胞增殖与示踪检测试剂盒（CFSE）

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流式细胞仪荧光酶标板荧光显微镜淋巴细胞增殖检测细胞增殖与示踪

Product no.40714ES Sizes2 options Documents1 files SourceYeasen · Official

Cat. No. 40714ES76

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40714ES76 500 T $378.00促~~$398.00~~ 现货 Enquire

40714ES80 1000 T $698.00促~~$728.00~~ 现货 Enquire

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Overview

Specifications

Brand翌圣生物 Yeasen

Cat.No40714ES

Specification本品为CFDA，SE细胞增殖与示踪检测试剂盒，包含配制CFDA，SE储存液所需的溶剂和细胞标记用的染色缓冲液，简化了实验前期准备工作。另，CFDA,SE标记细胞一般15 min即可完成，对于不同细胞，需要自行摸索最佳标记时间。按照每个样本的

Origin翌圣生物科技(上海)股份有限公司

技术平台 流式细胞仪 荧光酶标板 荧光显微镜

应用方法 淋巴细胞增殖检测最常用于淋巴细胞增殖 细胞增殖与示踪体外和体内增殖研究

检测方法 荧光检测

生物特性参数 试剂类别细胞增殖与示踪检测试剂盒 细胞系兼容性淋巴细胞、成纤维细胞、NK细胞、造血祖细胞等 作用机制被动运输进入细胞，被酯酶催化生成CFSE，与氨基结合，荧光随分裂减半 检测靶标细胞增殖 应用细胞增殖与示踪 标记对象活细胞 proliferation_detection_principle(增殖检测原理):荧光强度随分裂次数减半

样本类型细胞

技术参数 产品形式试剂盒 组分CFDA SE粉末、溶剂、染色缓冲液 激发波长494 nm 发射波长521 nm 储存条件-20℃干燥避光，有效期2年 运输条件冰袋运输 标记时间15 min 检测次数500次/1000次（不同货号） 标记体积2 mL/样本 荧光稳定性可达数月 可检测分裂次数多达8次或更多 检测通道FL1 荧光颜色绿色

实验类型 细胞增殖检测检测细胞分裂增殖 细胞示踪标记并追踪细胞

FAQ

Q：浓度是多少呢？

A：固体含量大概是1.4mg/管，溶解到B中浓度大概是2.8mg/mL。

Documents & Citations

细胞功能检测和探针整体方案 Open document

Published citations

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[3] Li X, Fu G, Zhang L, et al. Assay establishment and validation of a high-throughput organoid-based drug screening platform. Stem Cell Res Ther. 2022;13(1):219. Published 2022 May 26. doi:10.1186/s13287-022-02902-3(IF:6.832)

[4] He XT, Li X, Xia Y, et al. Building capacity for macrophage modulation and stem cell recruitment in high-stiffness hydrogels for complex periodontal regeneration: Experimental studies in vitro and in rats. Acta Biomater. 2019;88:162-180. doi:10.1016/j.actbio.2019.02.004(IF:6.638)

[5] Yang D, Zhang S, Fang X, et al. N-Benzyl/Aryl Substituted Tryptanthrin as Dual Inhibitors of Indoleamine 2,3-Dioxygenase and Tryptophan 2,3-Dioxygenase. J Med Chem. 2019;62(20):9161-9174. doi:10.1021/acs.jmedchem.9b01079(IF:6.054)

[6] Song J, He Z, Yang M, et al. HepaticIschemia/Reperfusion Injuryinvolves functional tryptase/PAR-2 signaling in liver sinusoidal endothelial cell population. Int Immunopharmacol. 2021;100:108052. doi:10.1016/j.intimp.2021.108052(IF:4.932)